Protein Info for PS417_24105 in Pseudomonas simiae WCS417

Updated annotation (from data): D-lactate transporter (lctP family)
Rationale: specific phenotype for D-lactate and no other transporter for D-lactate was apparent in the fitness data. Also important for utilization of D,L-lactate. Not clear if it transports L-lactate as well or not, but since it is in an apparent operon with a D-lactate dehydrogenase (PS417_24110), its main role is probably for transport of D-lactate.
Original annotation: L-lactate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 transmembrane" amino acids 13 to 35 (23 residues), see Phobius details amino acids 42 to 61 (20 residues), see Phobius details amino acids 72 to 95 (24 residues), see Phobius details amino acids 116 to 149 (34 residues), see Phobius details amino acids 153 to 180 (28 residues), see Phobius details amino acids 199 to 217 (19 residues), see Phobius details amino acids 223 to 242 (20 residues), see Phobius details amino acids 252 to 269 (18 residues), see Phobius details amino acids 276 to 297 (22 residues), see Phobius details amino acids 310 to 330 (21 residues), see Phobius details amino acids 335 to 354 (20 residues), see Phobius details amino acids 381 to 399 (19 residues), see Phobius details amino acids 419 to 438 (20 residues), see Phobius details amino acids 444 to 468 (25 residues), see Phobius details amino acids 540 to 559 (20 residues), see Phobius details TIGR00795: transporter, lactate permease (LctP) family" amino acids 7 to 556 (550 residues), 743.4 bits, see alignment E=8.3e-228 PF02652: Lactate_perm" amino acids 16 to 552 (537 residues), 699.7 bits, see alignment E=1.3e-214

Best Hits

KEGG orthology group: K03303, lactate transporter, LctP family (inferred from 99% identity to pfs:PFLU5278)

Predicted SEED Role

"L-lactate permease" in subsystem Lactate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U1J6 at UniProt or InterPro

Protein Sequence (564 amino acids)

>PS417_24105 D-lactate transporter (lctP family) (Pseudomonas simiae WCS417)
MQTWQQLYSPLGSLGLSAMAAVIPIVFFFLALAVFRLKGHVAGSITLALSILVAIFAFQM
PVDMALAAAGYGFAYGLWPIAWIIVAAVFLYKLTVKSGQFEIIRSSVLSITDDQRLQVLL
IGFCFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLCLIANTAPVAFGALGIPIIVA
GQVTGIDAFKIGAMTGRQLPLLSLFVPFWLVFMMDGVRGVRETWPAALVAGLSFAITQYF
TSNFIGPELPDITSALASLISLTLFLKVWQPKRTAGAQIAGATSSAAVTASAGGFGLPRN
TIVSPYSLGQIFKAWSPFLILTVLVTIWTLKPFKAMFAAGGSMYSWVFNFAIPHLDQLVI
KVAPIVTNPTAIPAVFKLDPISATGTAIFFSALISMLVLKIDIKTGLTTLKETFYELRWP
ILSIGMVLAFAFVTNYSGMSSTMALVLAGTGAAFPFFSPFLGWLGVFLTGSDTSSNALFS
SLQATTAHQIGVNDTLLVAANTSGGVTGKMISPQSIAVACAATGLVGKESDLFRFTLKHS
LFFATIVGLITLAQAYWFTGMLVH