Protein Info for PS417_24060 in Pseudomonas simiae WCS417

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 TIGR02350: chaperone protein DnaK" amino acids 3 to 603 (601 residues), 944.9 bits, see alignment E=8.2e-289 PF00012: HSP70" amino acids 4 to 603 (600 residues), 874.4 bits, see alignment E=5.3e-267 PF06723: MreB_Mbl" amino acids 116 to 377 (262 residues), 42.4 bits, see alignment E=6.3e-15

Best Hits

Swiss-Prot: 99% identical to DNAK_PSEFS: Chaperone protein DnaK (dnaK) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 99% identity to pfs:PFLU5269)

MetaCyc: 75% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UHK0 at UniProt or InterPro

Protein Sequence (640 amino acids)

>PS417_24060 molecular chaperone DnaK (Pseudomonas simiae WCS417)
MGKIIGIDLGTTNSCVSVMENGKAKVIENAEGARTTPSIIAYANDGEILVGQSAKRQAVT
NPHNTLYAVKRLIGRKFDEEVVQKDIKMVPYKIAKADNGDAWVEVNGNKMSAPQISAEVL
KKMKKTAEDYLGETVTEAVITVPAYFNDSQRQATKDAGRIAGLDVKRIINEPTAAALAYG
MDKAKGDHTVIVYDLGGGTFDVSVIEIAEVDGEHQFEVLATNGDTFLGGEDFDIRLIDYL
VDEFKKESGMNLKGDPLAMQRLKEAAEKAKIELSSSMSTDVNLPYITADATGPKHLNVKI
SRAKLEALVEDLVQRTIEPCRIALKDAGIDVGSINDVILVGGQTRMPLVQQKVTEFFGKE
ARKDVNPDEAVAMGAAIQGAVLAGDVKDVLLLDVSPLTLGIETMGGVMTALIEKNTTIPT
KKSQVFSTADDNQGAVTIHVLQGERKQAAQNKSLGKFDLSEIPPAPRGVPQIEVTFDIDA
NGILHVGAKDKATGKEQKITIKANSGLSDEEIQQMIRDAEANADADAKFAELAGARNQGD
ALVHSTRKMVADAGDKVTAEEKTAIEAAVVALEAAVKGDDKAAIEAKIEELSKVSAPVAQ
KMYAEQGQPADGAAQQAEPEAKHDDVVDAEFEEVKEDQKK