Protein Info for GFF4689 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Acetyl-coenzyme A synthetase (EC 6.2.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 49 to 389 (341 residues), 208.7 bits, E=1.3e-65
• PF13193: AMP-binding_C
  • amino acids 455 to 533 (79 residues), 70 bits, E=2.6e-23

Best Hits

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 78% identity to mpt:Mpe_A0343)

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• chitin deacetylation (2/4 steps found)
• cis-geranyl-CoA degradation (4/9 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (4/9 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1

Use Curated BLAST to search for 6.2.1.1

Protein Sequence (566 amino acids)

>GFF4689 Acetyl-coenzyme A synthetase (EC 6.2.1.1) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTASTASFLAARDFLLTHRQDYERAYRDFQWPVLDEFNWALDHFDPMARGNDAPALHIVG
EDGSEVKRSFAQMAERSSQVANHLRGLGVKRGERILLMLGNELPLWEVMLAAMKLGAVLI
PATALLTADDLRDRLERGQVRHVVAASAQTGKFEGLAGDYTRISVGEGVPGWRRFEDAAT
AATAFTPDGPTRATDPLLLYFTSGTTSKPKLVLHTHQSYPVGHLATMYWIGLLPGDVHLN
ISSPGWAKHAWSCFFAPWNAGACIFIYNYARFDAPGLLSVLERHRVTSLCAPPTVWRMLI
QEDLAARRGRLSLREVIGAGEPLNPEIIEQVQAAWGLSLRDGYGQTETTAQIGNPPGQRL
KPGSMGRPLPGYRIDLLDVDGQPASEGEVSLPLAQRPLGLMAGYQGNPSQNEDAMRDGYY
HTGDIAQRDADGYITFVGRADDVFKASDYRISPFELESALMEHEAVMEVAVVPSPDPVRL
AVPKAYLILRPGVQAGPELAQAIFAFSREHLAPYKRIRRLEFVQELPKTISGKIRRVQLR
TEESQRVAAGQRGGGAFHEEDFPARP