Protein Info for GFF4671 in Variovorax sp. SCN45

Annotation: putative membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details amino acids 61 to 81 (21 residues), see Phobius details amino acids 102 to 134 (33 residues), see Phobius details amino acids 141 to 163 (23 residues), see Phobius details amino acids 205 to 226 (22 residues), see Phobius details amino acids 246 to 264 (19 residues), see Phobius details amino acids 276 to 300 (25 residues), see Phobius details amino acids 320 to 339 (20 residues), see Phobius details amino acids 345 to 365 (21 residues), see Phobius details PF04235: DUF418" amino acids 227 to 386 (160 residues), 147.8 bits, see alignment E=1.5e-47

Best Hits

KEGG orthology group: K07148, uncharacterized protein (inferred from 66% identity to vap:Vapar_3571)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (403 amino acids)

>GFF4671 putative membrane protein (Variovorax sp. SCN45)
MSTTAPARERQSLVDALRGFALLGILVVNIATFASPFYGMGVPDPMAQSAAERAALFMRS
FLFETKFYLLFSFLFGYSFTLQMDSAERDGKAFAPRMLRRLLGLWVLGLLHAVVLYHGDI
LTTYAAIGGVLLLLRRRGDGFMAFCAVALVLVTSLLWASLGYLMTRAGIDADTRSLHAEA
MAALHAYRGTPATVIAQHLRELAQVWVVLALAQAPTALAMFLAGFIAGRRGLLANAAAHR
TLFKRLVICGLLIGLPGAVVYGLSTMRVGDGGREVVGFGLGLLTAPFLTAAYASALLLFF
QTPRGQGLVAWLAPAGRMALSNYLLQSLVCAWIFLAYGLRLIGSVGPLGGFAFAFSIFAA
QLVLSRWWMGRFAYGPVEWLLRAFTLLEWPSMRKAQRVKSVES