Protein Info for GFF4656 in Variovorax sp. SCN45

Annotation: TonB-dependent siderophore receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 signal peptide" amino acids 1 to 54 (54 residues), see Phobius details PF07715: Plug" amino acids 186 to 291 (106 residues), 67.9 bits, see alignment E=1.1e-22 TIGR01783: TonB-dependent siderophore receptor" amino acids 188 to 850 (663 residues), 337.9 bits, see alignment E=6.9e-105 PF00593: TonB_dep_Rec" amino acids 389 to 818 (430 residues), 178.9 bits, see alignment E=3.4e-56

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 72% identity to del:DelCs14_4743)

Predicted SEED Role

"TonB-dependent siderophore receptor" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (850 amino acids)

>GFF4656 TonB-dependent siderophore receptor (Variovorax sp. SCN45)
MSDVTLSPISPARRKAGPLLPTRKATLALAALLACGSTASFAQTPSAAPGATAATDGAME
WNLPADELGLVLARIARQGGRTISAAPSLVAGRRAQAVQGRFTVAQAAQQALTGSGLALA
QTPSGTLTVVASSGNASASATPEAAGTTLAEVRVTAQAERTGTTEGTGAYTARGPSTSAT
GLGLSLKETPQSISVITQQRMEDENLTSIKQVLARTPGLSTSTLGTERTSASARGYPITN
YQLDGISTHSEFLGLDALPSQSIADMALYDRIEVLRGASGLTTGAGDPSGIINMVRKKPT
ASFQGLAEASVGSWGNRRGMLDISTPLNESGSVRGRMVAVHEEGNSYIDYYRKKKDVFYG
VVEADLTSTLKLTAGIDHQENRSRGSLSYLGYPLFNSSGEQVDLPVSFSGASRDNRFNTN
STSGFLTLEQTLANDWKLKLSANYLQSKQREDSVYLAVNSSLFDKFTGDGLNLNAERRDY
DLRNKTVDLKLTGPFTLFGRQHEAVIGADYTDFRSLTNGSFDISGLRNAPVNIYRWNNAG
TPVFGERFVTFDSTRRQKSIYGAGRFQLSDQFKLVVGGKLLNYDSDYITKTTDGYDSKSP
SSERRVFTPYAGLLYDLDENHTLYTSFATIYNPQSSIDRYGALLDPQKGDTYEAGIKSSF
LGGRVTSSAAVYQIKQDNIAEADSGYFVPGTTNTASRAIKGATTRGIDLELNGTLARGWN
VAASYNFSTSKDATGQRINTTFPRQMARLWTTYRLPGDWRRLTLGGGVDWSSGFYYTGEA
WQIERTVTARQGAYAVASLMARYDVNDQLSVTLNVQNLFDRKYISSVSGWWYSGTYGAPR
NVQLTARYKF