Protein Info for PS417_23810 in Pseudomonas simiae WCS417

Annotation: acetolactate synthase 3 catalytic subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00118: acetolactate synthase, large subunit, biosynthetic type
  • amino acids 4 to 568 (565 residues), 849.6 bits, E=5.2e-260
• PF02776: TPP_enzyme_N
  • amino acids 5 to 118 (114 residues), 147.6 bits, E=2e-47
• PF00205: TPP_enzyme_M
  • amino acids 196 to 330 (135 residues), 159.9 bits, E=4.6e-51
• PF02775: TPP_enzyme_C
  • amino acids 396 to 545 (150 residues), 182.7 bits, E=5.9e-58

Best Hits

Swiss-Prot: 63% identical to ILVI_ECOLI: Acetolactate synthase isozyme 3 large subunit (ilvI) from Escherichia coli (strain K12)

KEGG orthology group: K01652, acetolactate synthase I/II/III large subunit [EC: 2.2.1.6] (inferred from 100% identity to pfs:PFLU5220)

MetaCyc: 63% identical to acetolactate synthase / acetohydroxybutanoate synthase, catalytic subunit (Escherichia coli K-12 substr. MG1655)
Acetolactate synthase. [EC: 2.2.1.6]; 2.2.1.6 [EC: 2.2.1.6]

Predicted SEED Role

"Acetolactate synthase large subunit (EC 2.2.1.6)" in subsystem Acetoin, butanediol metabolism or Branched-Chain Amino Acid Biosynthesis (EC 2.2.1.6)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-valine biosynthesis (4/4 steps found)
• pyruvate fermentation to (R)-acetoin I (3/3 steps found)
• pyruvate fermentation to (S)-acetoin (3/3 steps found)
• superpathway of 2,3-butanediol biosynthesis (5/6 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• superpathway of (R,R)-butanediol biosynthesis (4/5 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• pyruvate fermentation to (R)-acetoin II (1/2 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• superpathway of L-threonine metabolism (12/18 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.6

Use Curated BLAST to search for 2.2.1.6

Sequence Analysis Tools

See A0A1N7UNH7 at UniProt or InterPro

Protein Sequence (574 amino acids)

>PS417_23810 acetolactate synthase 3 catalytic subunit (Pseudomonas simiae WCS417)
MELLSGGEMLVRFLRDEGVDYIYGYPGGALLHVYDALFKEPAVTHILVRHEQAATHMADG
YARATGKAGVVLVTSGPGATNAITGIATAYMDSIPMVIISGQVPSTMVGTDAFQETDMIG
ISRPIVKHSFMIKHPSEIPEVMKKAFYLAQSGRPGPVVVDVPKDMTNPAEKFEYIFPKKA
KLRSYSPAVRGHSGQIRKAAEMLLAAKRPVLYSGGGVILGGGSAPLTELAKLLNLPVTNT
LMGLGAFPGTDRQFVGMLGMHGSYTANLAMHHADVILAVGARFDDRVINGASKFCPNAKI
IHIDIDPASISKTIKADVPIVGPVESVLTEMVAALKDIGETPNKESVASWWKQIDEWRGD
RGLFPYDKGDGSIIKPQTVIETLCEVTKGDAFVTSDVGQHQMFAAQYYKFDKPNRWINSG
GLGTMGFGFPAAMGVKLSFPDTDVACVTGEGSIQMNIQELSTCLQYGLPVKIVCLNNGVL
GMVRQWQDMSYNSRHSHSYMESLPDFVKLVEAYGHVGIRITDLKDLKPKMEEAFAMKDRL
VFIDIQVDTSEHVYPMQIKDGSMRDMWLNKTERT