Protein Info for GFF4648 in Variovorax sp. SCN45

Annotation: Inner-membrane proton/drug antiporter (MSF type) of tripartite multidrug efflux system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 510 transmembrane" amino acids 14 to 35 (22 residues), see Phobius details amino acids 51 to 74 (24 residues), see Phobius details amino acids 81 to 104 (24 residues), see Phobius details amino acids 110 to 129 (20 residues), see Phobius details amino acids 140 to 161 (22 residues), see Phobius details amino acids 167 to 189 (23 residues), see Phobius details amino acids 201 to 218 (18 residues), see Phobius details amino acids 238 to 258 (21 residues), see Phobius details amino acids 269 to 290 (22 residues), see Phobius details amino acids 306 to 327 (22 residues), see Phobius details amino acids 334 to 350 (17 residues), see Phobius details amino acids 362 to 388 (27 residues), see Phobius details amino acids 401 to 419 (19 residues), see Phobius details amino acids 476 to 493 (18 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 15 to 486 (472 residues), 403.6 bits, see alignment E=6.3e-125 PF07690: MFS_1" amino acids 19 to 411 (393 residues), 205.1 bits, see alignment E=1.5e-64 PF00083: Sugar_tr" amino acids 25 to 192 (168 residues), 41.5 bits, see alignment E=8.8e-15

Best Hits

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 58% identity to bgf:BC1003_2887)

Predicted SEED Role

"Multidrug resistance protein B"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (510 amino acids)

>GFF4648 Inner-membrane proton/drug antiporter (MSF type) of tripartite multidrug efflux system (Variovorax sp. SCN45)
MSVAPGTKVPHRGMITISIMLATIMQALDTTIANVALPHMQGSLQASQDQITWVLTSYIV
ASAIALPLTGWLCGHWGRRRVFIISVIGFTVASALCGLATSLVGIVAARLLQGIFGAALV
PLSQAVLLDINPREKVGQAMAIWGAGIMVGPILGPLLGGWLTENFDWRWVFFINLPVGIF
AFWGIARYLPESRPQGEKLDIFGFVTLSLAIGMLQLFLDRGEQLDWFDSWEIKLEAAAAL
VAFGFFVVHTWTVQGVSFFDRDLLKDRNFVTGLLFAFIVGMILFATMALLPNFLQGLMGY
PVVYTGQVTAPRGVGTMIAMIIVGRLVQKVDVRAIMAVGFGLTAFSLYQMTRLTLQMDST
LIIVSGFIQGLGIGFTFVPLSTATFATLAPRLRNQGTPIFSLLRNIGSSVGISIVQALLT
EGSSKAHAQIAATVAPGNQALSTLPQMMSPDTMTGLALLNAEVTRQGALVGYLDDFAIMM
IITVLAIPLLLLIRSPKRSAAASGPAEVPH