Protein Info for GFF4643 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Sirohydrochlorin cobaltochelatase (EC 4.99.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01903: CbiX
  • amino acids 11 to 114 (104 residues), 81.3 bits, E=5.9e-27
  • amino acids 139 to 243 (105 residues), 71.8 bits, E=5.6e-24

Best Hits

KEGG orthology group: K03795, sirohydrochlorin cobaltochelatase [EC: 4.99.1.3] (inferred from 82% identity to pna:Pnap_2162)

Predicted SEED Role

"Sirohydrochlorin cobaltochelatase (EC 4.99.1.3)" in subsystem Coenzyme B12 biosynthesis or Experimental tye (EC 4.99.1.3)

MetaCyc Pathways

• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (13/15 steps found)
• siroheme biosynthesis (3/4 steps found)
• factor 430 biosynthesis (3/7 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.99.1.3

Use Curated BLAST to search for 4.99.1.3

Protein Sequence (317 amino acids)

>GFF4643 Sirohydrochlorin cobaltochelatase (EC 4.99.1.3) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNTETILLVGHGSREASGNDEIFAFAEQWRQRQPGWRIEVCFIEFSEITMSEGLRRAATG
ASRVMVVPLILNAAGHVKMDIPQAIEGARLKFPLVQFLYAPHLSACDPILAILRRRLKGA
MQALDMPDPSTTGVVVLGRGSSDRQANGDMAKMARWLLEETDHELVDLAFTGITHPRLEK
TVQRQSLLGMKQVVVLPYYLFNGTLVERITRQVEHLKSQYPMLRFASTKYFGFEKEIFEL
LEQRVNDLRRNAPVALMPCDGCKYRSFAEEHGMGGHHHDDALPHSHDHAHPHEHPHAPVH
AHDFAHHHEHPHDHARA