Protein Info for GFF4635 in Variovorax sp. SCN45

Annotation: Transketolase, N-terminal section (EC 2.2.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00456: Transketolase_N
  • amino acids 14 to 260 (247 residues), 122.7 bits, E=4.4e-39
• PF09364: XFP_N
  • amino acids 100 to 215 (116 residues), 27.6 bits, E=3.3e-10
• PF00676: E1_dh
  • amino acids 109 to 215 (107 residues), 41.9 bits, E=1.6e-14
• PF13292: DXP_synthase_N
  • amino acids 114 to 173 (60 residues), 29.6 bits, E=1.1e-10

Best Hits

KEGG orthology group: K00615, transketolase [EC: 2.2.1.1] (inferred from 64% identity to bac:BamMC406_2288)

MetaCyc: 39% identical to 6-deoxy-6-sulfo-D-fructose transketolase subunit A (Clostridium sp. MSTE9)
RXN-22969 [EC: 2.2.1.15]; 2.2.1.15 [EC: 2.2.1.15]

Predicted SEED Role

"Transketolase, N-terminal section (EC 2.2.1.1)" in subsystem Calvin-Benson cycle or Pentose phosphate pathway (EC 2.2.1.1)

MetaCyc Pathways

• Bifidobacterium shunt (15/15 steps found)
• superpathway of glucose and xylose degradation (15/17 steps found)
• pentose phosphate pathway (8/8 steps found)
• Rubisco shunt (9/10 steps found)
• pentose phosphate pathway (non-oxidative branch) I (5/5 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• pentose phosphate pathway (partial) (3/3 steps found)
• pentose phosphate pathway (non-oxidative branch) II (5/6 steps found)
• Calvin-Benson-Bassham cycle (10/13 steps found)
• formaldehyde assimilation II (assimilatory RuMP Cycle) (7/9 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
• oxygenic photosynthesis (11/17 steps found)
• sulfoquinovose degradation V (2/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of ansamycins
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Carbon fixation in photosynthetic organisms
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.1

Use Curated BLAST to search for 2.2.1.1 or 2.2.1.15

Protein Sequence (270 amino acids)

>GFF4635 Transketolase, N-terminal section (EC 2.2.1.1) (Variovorax sp. SCN45)
MKSQFDPSSLRKTVVEMAYAGNAVHLGCAFSIIELLSCLYGRHMRYDKLVPTDPMRDYLV
LSKGHGVMAQYACLYELGWLDRSDIDNYFHDGSRLKGLSDAHLPGCEVTSGTLGHGLSVG
VGLALAASRRKTGQKCYAVIGDGEINEGPIWEAMLFAAHAKLDNLMIIVDANGFQAMGTT
DEVMQLGNLNAKFSAFGFDVLAVDGHDMSAISSAIDELEAGRNGRPKALVAKTIKGKGVS
YMENVNAWHYSRMNADLYAQALRELDGAVA