Protein Info for PS417_23670 in Pseudomonas simiae WCS417

Updated annotation (from data): Muconate cycloisomerase (EC 5.5.1.1)
Rationale: Specifically important for utilizing L-Tryptophan. Automated validation from mutant phenotype: the predicted function (MUCONATE-CYCLOISOMERASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: muconate cycloisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02534: muconate and chloromuconate cycloisomerases
  • amino acids 6 to 373 (368 residues), 527.6 bits, E=8.7e-163
• PF02746: MR_MLE_N
  • amino acids 9 to 129 (121 residues), 128.3 bits, E=1.8e-41
• PF13378: MR_MLE_C
  • amino acids 151 to 365 (215 residues), 180.4 bits, E=4.3e-57

Best Hits

Swiss-Prot: 76% identical to CATB_PSEPU: Muconate cycloisomerase 1 (catB) from Pseudomonas putida

KEGG orthology group: K01856, muconate cycloisomerase [EC: 5.5.1.1] (inferred from 97% identity to pfs:PFLU5192)

MetaCyc: 90% identical to muconate cycloisomerase (Pseudomonas reinekei)
Muconate cycloisomerase. [EC: 5.5.1.1]

Predicted SEED Role

"Muconate cycloisomerase (EC 5.5.1.1)" in subsystem Catechol branch of beta-ketoadipate pathway or Muconate lactonizing enzyme family (EC 5.5.1.1)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• superpathway of salicylate degradation (7/7 steps found)
• catechol degradation III (ortho-cleavage pathway) (6/6 steps found)
• catechol degradation to β-ketoadipate (4/4 steps found)
• mandelate degradation to acetyl-CoA (9/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)

KEGG Metabolic Maps

• 1,4-Dichlorobenzene degradation
• Benzoate degradation via hydroxylation
• Fluorobenzoate degradation
• Toluene and xylene degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.5.1.1

Sequence Analysis Tools

See A0A1N7UBZ4 at UniProt or InterPro

Protein Sequence (375 amino acids)

>PS417_23670 Muconate cycloisomerase (EC 5.5.1.1) (Pseudomonas simiae WCS417)
MPICAIESIDTIIVDLPTLRPHKLAMHTMQNQTLVIIRLRCADGIEGIGEATTIGGLSYG
NESPDSIKVNIDRHFAPLLIGQDASNINAAMLRLERSIRGNTFAKSGIESALLDALGKRL
NLPVSELLGGRVRDALPVAWTLASGNTEKDIAEAEKMLDLRRHRLFKLKIGAGEVSHDLA
HVIAIKKALGDRASVRVDVNQAWDEAVALRACKVLGDNGIDLIEQPISRNNRGGMARLNL
SSPAPIMADESIECVEDAFNLAREGAASVFALKIAKNGGPRAVLRTAAIAEAAGIGLYGG
TMLEGGIGTLASAHAFLTLNKLAWDTELFGPLLLTEDILTEPPVYRDFQLHVSTAPGLGL
AIDEERLAFFRRDKH