Protein Info for GFF4626 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 18 to 86 (69 residues), 83.8 bits, E=5.9e-28
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 137 to 593 (457 residues), 706.9 bits, E=4.1e-217
• PF01964: ThiC_Rad_SAM
  • amino acids 138 to 589 (452 residues), 621.2 bits, E=8.6e-191

Best Hits

Swiss-Prot: 86% identical to THIC_LEPCP: Phosphomethylpyrimidine synthase (thiC) from Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 86% identity to lch:Lcho_2430)

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

Compare fitness of predicted isozymes for: 4.1.99.17

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (635 amino acids)

>GFF4626 Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNAPDQLPVSTLALSREPFPASRKLYVPGQQPGVRVPMREIALTNGERVVVYDTSGPYSE
PSTMIDVTRGLPDVRGAWIASRGDTETYAGRARQVLDDGVKNEARQNDTTIERIEALRAQ
AAGLQRTPRRAKSGMNVTQMHYARRGIVTPEMEYVAVRENGKREWMAEYLGDTAREARLR
GNPMGARIPDIITPEFVRDEVARGRAIIPANINHPEVEPMAIGRNFLVKVNANIGNSAVT
SSIEEEVDKLVWAIRWGADNVMDLSTGRNIHTTRDWIVRNSPVPIGTVPIYQALEKVGGV
AEDLTWAIYRDTLIEQAEQGVDYFTIHAGVRLAYIHLTANRRTGIVSRGGSIMAKWCITH
HRESFLYENFDEICEIMKAYDVSFSLGDGLRPGSAADANDEAQFAELKTLGELTQTAWKH
DVQTMIEGPGHVPMHLIQANMDEQLKHCHEAPFYTLGPLTIDIAPGYDHIASAIGAAMIG
WMGTAMLCYVTPKEHLGLPDRDDVKQGLIAYKIAAHAADIAKGHPGARARDDAMSKARFE
FRWHDQFNLGLDPDTAREFHDETLPKDSSKEAHFCSMCGPKFCSMKITQDVRDYAEKQGL
SETDVLARGMAAKSAEFKAAGGAFYVPVDTLTRKA