Protein Info for GFF4615 in Variovorax sp. SCN45

Annotation: 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 67% identical to UGL_BURCJ: Ureidoglycolate lyase (BceJ2315_61450) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: None (inferred from 94% identity to vpe:Varpa_0813)

MetaCyc: 66% identical to 5-hydroxy-2,4-dioxopentanoate hydrolase (Herbaspirillum huttiense)
RXN-12096 [EC: 3.7.1.26]; 3.7.1.26 [EC: 3.7.1.26]

Predicted SEED Role

"5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase." in subsystem Aromatic amino acid degradation

MetaCyc Pathways

L-arabinose degradation V (5/6 steps found)
L-fucose degradation III (5/8 steps found)
D-arabinose degradation IV (3/6 steps found)
D-xylose degradation VI (2/5 steps found)
L-rhamnose degradation III (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (282 amino acids)

>GFF4615 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase (Variovorax sp. SCN45)
MKLVRYGNPGKEKPGLIDADGKLRDLSAIVKDIGPEQLGDAAIAKLRKQKVDKLPLVKGK
PRFGSPVANVGKFIAIGLNYADHAAESGLPVPKEPVVFTKANSCIQGPNDPVMLPKGSVK
TDWEVELGVVIGTRARYVSQKSALDYVAGYCTINDISEREFQIERGGTWDKGKGCDTFGP
IGPWLVTRDEIENPQKLAMWLDLNGQRVQTGNTKTMIFSVAKIVSYVSQFMTLMPGDVIT
TGTPPGVGMGMKPPLFLKKGDVMTLGIEGLGEQRQEVIPFKL