Protein Info for GFF4580 in Pseudomonas sp. DMC3

Annotation: Glutathione-regulated potassium-efflux system protein KefC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 570 transmembrane" amino acids 6 to 22 (17 residues), see Phobius details amino acids 27 to 43 (17 residues), see Phobius details amino acids 57 to 77 (21 residues), see Phobius details amino acids 90 to 124 (35 residues), see Phobius details amino acids 144 to 166 (23 residues), see Phobius details amino acids 172 to 195 (24 residues), see Phobius details amino acids 214 to 248 (35 residues), see Phobius details amino acids 267 to 285 (19 residues), see Phobius details amino acids 291 to 313 (23 residues), see Phobius details amino acids 323 to 342 (20 residues), see Phobius details amino acids 354 to 374 (21 residues), see Phobius details TIGR00932: transporter, monovalent cation:proton antiporter-2 (CPA2) family" amino acids 10 to 281 (272 residues), 245.9 bits, see alignment E=2.8e-77 PF00999: Na_H_Exchanger" amino acids 10 to 370 (361 residues), 225.1 bits, see alignment E=1.3e-70 PF02254: TrkA_N" amino acids 405 to 518 (114 residues), 76.4 bits, see alignment E=2.3e-25

Best Hits

KEGG orthology group: K03455, monovalent cation:H+ antiporter-2, CPA2 family (inferred from 91% identity to pfo:Pfl01_3967)

Predicted SEED Role

"Glutathione-regulated potassium-efflux system protein KefB" in subsystem Glutathione-regulated potassium-efflux system and associated functions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (570 amino acids)

>GFF4580 Glutathione-regulated potassium-efflux system protein KefC (Pseudomonas sp. DMC3)
VFANLLIILASSLVVIALFRRLRLPPVLGYLCVGLLVGPNAFDWVNESEHLPDVAELGVV
FLLFSLGLEFSLSKMIALRQVVFRLGSQQVLISTALLGTLLMGLGMPLMPALLLGAGLSL
SSTAIVTKELGSLGEVFSSHGQNAVGVLLFQDVVAVLLLTLVPVFAGTSEQAWYWALPLT
LGKTVVLFFGLLLASRWLLPRLFHEVAASRSAELFVLLALVIVLLTAWLTHLLGLSPALG
AFLAGMLLGESHYRHQIEADIRPFRDILLGVFFVSIGMLIDLQLFVSHSLLIIGLTLGLM
LIKGIVVALLVKWCGSDSETAWRSGLALAQGGEFCFALMAQMQQNSLLPAEPGALLLAAT
FCSMLLTPLLLRAAPRIAAVLHRKPNQEAQIEQISALNADLDQHVVICGYGRVGQSIGRF
MGNAGQAYVALDNDPVRVQEAASAESDVHYGDSARGDLLTAVGLLRARLLVIAVDQSDVA
LRILREARRLNRQVPILVRTRDDSQSAELRAAGATEVVPELLESSLMLASHALIMLGLPA
HKVQEKVDQVRIDRYRLLHGFYPGANDEEI