Protein Info for GFF4571 in Pseudomonas sp. DMC3

Annotation: putative lipid kinase YegS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 305 PF00781: DAGK_cat" amino acids 6 to 125 (120 residues), 87.8 bits, see alignment E=4.5e-29 TIGR03702: lipid kinase YegS" amino acids 6 to 295 (290 residues), 450.3 bits, see alignment E=2.5e-139 TIGR00147: lipid kinase, YegS/Rv2252/BmrU family" amino acids 7 to 290 (284 residues), 191.6 bits, see alignment E=1.5e-60 PF19279: YegS_C" amino acids 147 to 286 (140 residues), 43.8 bits, see alignment E=2.4e-15

Best Hits

Swiss-Prot: 88% identical to YEGS_PSEPF: Probable lipid kinase YegS-like (Pfl01_3958) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K07029, (no description) (inferred from 88% identity to pfo:Pfl01_3958)

Predicted SEED Role

"Transcription regulator [contains diacylglycerol kinase catalytic domain]"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (305 amino acids)

>GFF4571 putative lipid kinase YegS (Pseudomonas sp. DMC3)
MSERRALLILHGKQALNEDVRAAVEAKREQGWELAVRVTWEAGDAQRLVDEALAAGYSKI
IAGGGDGTLRDIAEALAQHPQKASLVLLPLGTANDFSRAAGISLEPAEALELLDVQPSDI
DLGEVGGQIFLNMATGGFGSQVTANTSEDLKKVLGGAAYLFTGLTRFSELHAAYGELQGP
DFHWQGELLALGIGNGRQAGGGHVLCPEARVDDGLLDISILPAPQELVGTLKNLLSEGFG
LDNMFVRARLPWVEIKVAEGLSINLDGEPLEGDSLRFVAKPAALQVHLPKDSPLLGQQPS
ISHQG