Protein Info for GFF4502 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: L-asparaginase (EC 3.5.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00710: Asparaginase
  • amino acids 9 to 191 (183 residues), 185.1 bits, E=1e-58
• PF17763: Asparaginase_C
  • amino acids 220 to 314 (95 residues), 62.4 bits, E=3.8e-21

Best Hits

Swiss-Prot: 38% identical to ASPG_HELPY: Probable L-asparaginase (ansA) from Helicobacter pylori (strain ATCC 700392 / 26695)

KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 59% identity to adn:Alide_2631)

Predicted SEED Role

"L-asparaginase (EC 3.5.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.1)

MetaCyc Pathways

• L-asparagine degradation I (1/1 steps found)
• L-asparagine degradation III (mammalian) (2/3 steps found)
• superpathway of L-aspartate and L-asparagine biosynthesis (2/4 steps found)
• N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Cyanoamino acid metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.1

Protein Sequence (319 amino acids)

>GFF4502 L-asparaginase (EC 3.5.1.1) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VVEANQGRRLVILGTGGTIAGRASVAGDNVGYVAGQVGVADLVAAVPALAGLPLDVEQVA
QIDSKDMGLPVWQALAQRLQVHLDDPQVSAVIVTHGTDTLEETAFVLQHLLQPAKPVVLT
CAMRPASALVPDGPQNLADAVAVAQQAQAGVVVVCAGQVHGALEVAKVHTYRVDAFDSGD
AGPLGCVEEGMLRRFRAWGAPVARHAAWMERFLAAKALPRVELLTSHADADGGLVQAVMA
AGASRPVQGIVVAGTGNGTVHQALTEALKAAQAQGVRVVLATRCARGRVVPHAGLPFGHS
AGLSPVKARLALALDLLMD