Protein Info for GFF4482 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Oxygen-insensitive NAD(P)H nitroreductase (EC 1.-.-.-) / Dihydropteridine reductase (EC 1.5.1.34)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00881: Nitroreductase
  • amino acids 8 to 193 (186 residues), 98.1 bits, E=3.3e-32

Best Hits

Swiss-Prot: 100% identical to NFSB_SALTY: Oxygen-insensitive NAD(P)H nitroreductase (nfsB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K10679, nitroreductase / dihydropteridine reductase [EC: 1.-.-.- 1.5.1.34] (inferred from 97% identity to ses:SARI_02359)

MetaCyc: 88% identical to NAD(P)H-dependent nitroreductase NfsB (Escherichia coli K-12 substr. MG1655)
1.7.1.-; NAD(P)H dehydrogenase (quinone). [EC: 1.6.5.2]; 6,7-dihydropteridine reductase. [EC: 1.6.5.2, 1.5.1.34]

Predicted SEED Role

"Oxygen-insensitive NAD(P)H nitroreductase (EC 1.-.-.-) / Dihydropteridine reductase (EC 1.5.1.34)" in subsystem Pterin biosynthesis (EC 1.-.-.-, EC 1.5.1.34)

MetaCyc Pathways

• tetrahydropteridine recycling (2/2 steps found)
• L-phenylalanine degradation V (1/3 steps found)
• superpathway of photosynthetic hydrogen production (3/6 steps found)
• vitamin K-epoxide cycle (1/4 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Carotenoid biosynthesis - General
• Folate biosynthesis
• Insect hormone biosynthesis
• Nucleotide sugars metabolism
• Porphyrin and chlorophyll metabolism
• Puromycin biosynthesis
• Trinitrotoluene degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-, 1.6.5.2

Use Curated BLAST to search for 1.-.-.- or 1.5.1.34 or 1.6.5.2

Protein Sequence (217 amino acids)

>GFF4482 Oxygen-insensitive NAD(P)H nitroreductase (EC 1.-.-.-) / Dihydropteridine reductase (EC 1.5.1.34) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MDIVSVALQRYSTKAFDPSKKLTAEEADKIKTLLQYSPSSTNSQPWHFIVASTEEGKARV
AKSAAGNYTFNERKMLDASHVVVFCAKTAMDDAWLERVVDQEDADGRFATPEAKAANDKG
RRFFADMHRVSLKDDHQWMAKQVYLNVGNFLLGVAAMGLDAVPIEGFDAEVLDAEFGLKE
KGYTSLVVVPVGHHSVEDFNAGLPKSRLPLETTLTEV