Protein Info for GFF4479 in Variovorax sp. SCN45

Annotation: Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 584 transmembrane" amino acids 27 to 46 (20 residues), see Phobius details PF03717: PBP_dimer" amino acids 68 to 218 (151 residues), 82.2 bits, see alignment E=6.5e-27 PF00905: Transpeptidase" amino acids 260 to 556 (297 residues), 260.5 bits, see alignment E=2e-81

Best Hits

Swiss-Prot: 43% identical to PBP2_NEIGO: Probable peptidoglycan D,D-transpeptidase PenA (penA) from Neisseria gonorrhoeae

KEGG orthology group: K03587, cell division protein FtsI (penicillin-binding protein 3) [EC: 2.4.1.129] (inferred from 95% identity to vpe:Varpa_0973)

Predicted SEED Role

"Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)" in subsystem Bacterial Cell Division or Bacterial Cytoskeleton or Flagellum in Campylobacter or Peptidoglycan Biosynthesis (EC 2.4.1.129)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129

Use Curated BLAST to search for 2.4.1.129

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (584 amino acids)

>GFF4479 Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129) (Variovorax sp. SCN45)
MTRGNRSVRYTTSPLLASKTPVWRSKFIVAGIALGFVMLAGRAAYVQVLNNSFFQRQGEV
RFARTLEMPANRGRILDRNGLILASSVVAPSIWAIPEDIERDDPEVRAKLKQVAKLLEMP
QKDFDKKLEDEDKTFVWIKRQVDEPIAKQIAAMNLKGIYQRKEYKRQYPEGEAAAHVVGF
TNVEDSGQEGIELAFNKELAGKTGSRRVIKDRLGRVVEGVGELVPPLDGQDIQLSVDSKV
QFFAYQKLRDAVIARKAKAGSVVVLDSTTGEVLALANYPSYVPDKRQNLTGEQLRNRALT
DTFEPGSTMKPITVGMALEAGRVKPSTVIDTAPGRYQIGGFTISDTHNYGSLTVEGVIQK
SSNVGALKIAQKMTPHEMWDTYTALGYGQKPQIQFPGAVTGRLRPWKTWKPVEQATMAYG
YGLSASLFQMAHSYTSFAHDGSLIPVTILKNSEPPTGVRVFSPSNALAVRKMLQMAAGPG
GTGTRAQTVGYSVGGKSGTAHKQVGKGYASNKYRAWFTGMAPIEKPRIIVAVMIDEPTDG
QYFGGLAAAPVFSEVVQQTLRMMNVPPDLAVKPLVMTQGVDESF