Protein Info for GFF4474 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 906 transmembrane" amino acids 418 to 439 (22 residues), see Phobius details amino acids 446 to 455 (10 residues), see Phobius details amino acids 459 to 479 (21 residues), see Phobius details amino acids 485 to 509 (25 residues), see Phobius details PF02702: KdpD" amino acids 21 to 229 (209 residues), 322.4 bits, see alignment E=2.9e-100 PF00582: Usp" amino acids 249 to 372 (124 residues), 44 bits, see alignment E=8.3e-15 PF13493: DUF4118" amino acids 420 to 517 (98 residues), 69.4 bits, see alignment E=5.1e-23 PF00512: HisKA" amino acids 676 to 742 (67 residues), 51.1 bits, see alignment 2.9e-17 PF02518: HATPase_c" amino acids 790 to 898 (109 residues), 80.1 bits, see alignment E=4.3e-26

Best Hits

KEGG orthology group: K07646, two-component system, OmpR family, sensor histidine kinase KdpD [EC: 2.7.13.3] (inferred from 86% identity to adn:Alide_3009)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (906 amino acids)

>GFF4474 Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MGAENTGQADALIGELQRQSAGRLTVFLGAAPGVGKTYAMLARARELHRQGVDVVIGIIE
THGRSETQALLEGLPQLPRRKVEYQGRTLDEMDLDGLLARKPAIALVDELAHRNVPGSRH
ERRWQDVEELLDAGIDVYTTVNIQHLESLNDVVHQITGIRVSETVPDAVLERLRDIRLVD
LPARELIERLNQGKVYLPEQAAQALQAFFSPSNLTALRELAMQTVAEHVDADLRETRTAR
GLGDIAIQRHVLIAIDGKGQSEYLVRAGARIAERRGAPWSVVAVDTGHSADAALHAEDLE
RAVAQRHSLAHTEQQRQMELDKAFALARNLGGDTEILYNTDIPQALLDAAAARGARSIVI
GRTRERPIARIFNRTLTQQLLLRGARYELTIVSTPQARQRARRFQDLAGERLAKGEPVLI
ALATAGAIAAAVVAERFLGLEDLSTVFLVAVLLVASRTRMAAAVITAVLCFLAYNFFFID
PRFTFLISAPRGVATVMLFMAAALIAGRLASRLRMQVISLRTANTHTTAMQNLARQLSKA
ADLGQVISASASVLQSSLNAQAWVRINGESGPSAVAGELTEKDLIAADWAQRHGQPSGRY
TDTLGQSTWWFLPIRSEQDTLGVVGLHFPSTMGRLSFEQRRLAESMTDDIGQAALRARLV
SELEVAKVTGETERLRSALLSSVSHDLRSPLSSMIGAADSLARYGKDMSADDRSDLLQTI
RVEGERLDRYIQNLLDMTRLGQQGLTLTRDWIGVDELVGSAARRLQRYEPSVKVEIDIDA
VVGLIHVHPALIEQALFNVMENAAKFSPPDEPIRVQARLAEGPAVQIDISDRGPGIPADE
RRRIFDMFYSVERGDRGRQGTGLGLTIVQGIVGAHMGKVEALPGPQGRGTSVRLTLPKGE
QSIEEV