Protein Info for GFF445 in Pseudomonas sp. DMC3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1179 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details transmembrane" amino acids 48 to 71 (24 residues), see Phobius details amino acids 448 to 468 (21 residues), see Phobius details TIGR03348: type VI secretion protein IcmF" amino acids 18 to 1176 (1159 residues), 1199.8 bits, see alignment E=0 PF14331: IcmF-related_N" amino acids 198 to 452 (255 residues), 276.1 bits, see alignment E=5.3e-86 PF06761: IcmF-related" amino acids 502 to 792 (291 residues), 278.7 bits, see alignment E=1.4e-86 PF06744: IcmF_C" amino acids 1054 to 1159 (106 residues), 86.3 bits, see alignment E=3.1e-28

Best Hits

KEGG orthology group: K11891, type VI secretion system protein ImpL (inferred from 95% identity to pfo:Pfl01_5577)

Predicted SEED Role

"IcmF-related protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1179 amino acids)

>GFF445 hypothetical protein (Pseudomonas sp. DMC3)
MKKFFKKVGAFLRQTWVWTLLLVLFVALLVWFVGPLLAVDDYKFWESATSRLLTISVLFL
IWGLTMVFVSWRAGIRKKAEEESEDGQDRIRREELIDEEQKELKARFKDALKTLKTSSLY
RGRSERWRSDLPWYLLIGPQASGKTSLLDFSGLEFPINKIDRKLTRDTLGTRHCDWYFAD
HGVLIDTAGRYTTQPDAEVDGNAWTTLLELLRKRRRGRPLNGVLVTIPVETLTGGSEQDI
ETLARQVRSRLQDVHQKLHVDVPVYLVLSKADKLLGFDEFFDQLTREESDQVLGTSFRKD
QVGTDVTVLRNEFEELLRRLNSQVIMRMHSERDTQRRGRILDFPHQLGQIGERLCLFVDM
AFTGNRYQRATQLRGFYLTSAPHLTQEMDSTTAGIGASLGMNAGVLPTLRSGRSRFIHHL
LSQVIFPEADLAGLDKRERSRIHWGQRALYVGALAALALFGMLWAGGFSANYERLENLRT
LAQNWTQQRSAVTPRDDAMGVLKVLDTSYAATQVFPNKGDVSYHERGGLYQGEEINPVVK
TAYERELEAQLLPRVATMLEGQIRANMKDRDRLLNSLRAYLMLNMKDRRDAAWLKDWVAT
DWSQRYTGNTAVQNGLNTHLERLLKQPFIYPLNDQLVTQARQVLRSESLANVVYRMLREQ
ARNLPDYRFSQHLGPQGSLFIGTEYVIPGFYTQQGYQQYFSVQGAALVTDILRDNWVLGE
GAGISDMDLRRLMVELEQLYFRDYANYWSEAVGQVALPPISDAGEGAEQLAGLTSANSPV
LALLTEVRENTRFEAAADPVDEAGEAADALAGQKGKLGKVGKLAAAAADKASALNVAKNL
PDTAKKSLQRRFEPLHRLLDDNNGPGADLTPAFSALNDLQLQLAGLARSSTPEQAAFEMA
KTRMSGQRDALTNLRNASGRLPRPLSVWFNVLAEDSWRLVLNDAYQYLNGRYQNELYSVY
GKTISKRYPFSASSTSDVAISDFREFFRAQGTVDRFFDSYMRPFVSGDPGNYRMRSVDGH
SLPVSKVYLDQMAAAQTIRQSFFSINPAEPTVQFKLEPYTLDPAVSRSEFKFGDKTMEYR
HGPILPMTFKWPTDAEDGRTSLVMDKMAGRPIGIEKNSGPWSLFRLFDLMQTEYLNGRDV
LVLKADVGGLRANYLLTSQRTPNPFDMGVLRTFRMPVQL