Protein Info for GFF4447 in Pseudomonas sp. DMC3

Annotation: putative lipoprotein YiaD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 244 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 45 to 61 (17 residues), see Phobius details amino acids 68 to 86 (19 residues), see Phobius details PF00691: OmpA" amino acids 121 to 216 (96 residues), 77.6 bits, see alignment E=3.9e-26

Best Hits

Swiss-Prot: 45% identical to YIAD_ECOLI: Probable lipoprotein YiaD (yiaD) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 93% identity to pba:PSEBR_a4661)

Predicted SEED Role

"Outer membrane protein assembly factor YaeT precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (244 amino acids)

>GFF4447 putative lipoprotein YiaD (Pseudomonas sp. DMC3)
MFTTRRLIIVATAVAVLSGCASPNPYDNQGQADGGSQGMSKTAKYGGLGALAGALAGAAI
GHDNRGKGALIGAAVVGASAAGYGYYADQQEKKLRASMANTGVEVQRQGDQIKLIMPGNI
TFATDSANIASSFYQPLNNLANSLKEFNQNQIEIVGYTDSTGSRQHNMDLSQRRAQSVAT
YLTSQGVSGANLSARGAGPDNPIASNGDVNGRAQNRRVEVNLKAIPGQQYGGQQQQPGTV
QQYP