Protein Info for GFF4441 in Variovorax sp. SCN45

Annotation: Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 109 (109 residues), 152.8 bits, E=1.4e-48
• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 1 to 444 (444 residues), 741.1 bits, E=2.2e-227
• PF02655: ATP-grasp_3
  • amino acids 114 to 294 (181 residues), 24.9 bits, E=5.8e-09
• PF02786: CPSase_L_D2
  • amino acids 115 to 322 (208 residues), 261.1 bits, E=2.1e-81
• PF07478: Dala_Dala_lig_C
  • amino acids 141 to 291 (151 residues), 35.5 bits, E=2.2e-12
• PF02222: ATP-grasp
  • amino acids 143 to 293 (151 residues), 31.9 bits, E=3e-11
• PF02785: Biotin_carb_C
  • amino acids 336 to 442 (107 residues), 130.7 bits, E=7.3e-42

Best Hits

Swiss-Prot: 70% identical to ACCC_ALLVD: Biotin carboxylase (accC) from Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 98% identity to vpe:Varpa_1007)

MetaCyc: 67% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• 3-hydroxypropanoate cycle (9/13 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (11/18 steps found)
• glyoxylate assimilation (7/13 steps found)
• jadomycin biosynthesis (3/9 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Protein Sequence (449 amino acids)

>GFF4441 Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14) (Variovorax sp. SCN45)
MFKKILVANRGEIALRIQRACSELGIKAVMVYSEADRDAKYIKLAEEAVCIGPAPSAQSY
LNMPAIISAAEVTDAEAIHPGYGFLSENANFAERVEQSGFQFIGPTPDNIRTMGDKVSAK
QAMIKAGVPCVPGSEGELSDDAATNKRIARAIGYPVIIKAAGGGGGRGMRVVHTEAALVN
AIQMTKAEAGAAFNNAAVYMEKFLQNPRHIEIQILADKHKNAVYLGERDCSMQRRHQKVI
EESPAPGIPRKLIEKIGERCAAACKKIGYRGAGTFEFLYENGEFYFIEMNTRVQVEHPVT
EFTTGIDIVKTQIMVAAGEKLPFTQRQIQMHGHAIEVRINAEDAWKFTPSPGRITMWHPP
GGPGVRVDSHAYTNYFVPPNYDSMIGKIIVYGDTREQAMARMRTALNETVIEGIQTNIPL
HRELMVDSKFMTGGTNIHYLEEWLAAHKR