Protein Info for GFF444 in Variovorax sp. SCN45

Annotation: Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ Cob(I)alamin adenosyltransferase (EC 2.5.1.17), clustered with cobalamin synthesis

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00708: cob(I)yrinic acid a,c-diamide adenosyltransferase
  • amino acids 18 to 188 (171 residues), 199.9 bits, E=1.4e-63
• PF02572: CobA_CobO_BtuR
  • amino acids 20 to 188 (169 residues), 206.7 bits, E=1.5e-65

Best Hits

Swiss-Prot: 46% identical to BTUR_ECOLI: Corrinoid adenosyltransferase (btuR) from Escherichia coli (strain K12)

KEGG orthology group: K00798, cob(I)alamin adenosyltransferase [EC: 2.5.1.17] (inferred from 97% identity to vpe:Varpa_3373)

Predicted SEED Role

"Cob(I)alamin adenosyltransferase (EC 2.5.1.17)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 2.5.1.17)

MetaCyc Pathways

• superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
• adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (6/6 steps found)
• superpathway of adenosylcobalamin salvage from cobinamide II (8/9 steps found)
• adenosylcobalamin salvage from cobalamin (5/5 steps found)
• adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
• adenosylcobinamide-GDP salvage from cobinamide II (5/6 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (23/33 steps found)
• cobalamin salvage (eukaryotic) (4/8 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (22/36 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.17

Use Curated BLAST to search for 2.5.1.17

Protein Sequence (189 amino acids)

>GFF444 Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ Cob(I)alamin adenosyltransferase (EC 2.5.1.17), clustered with cobalamin synthesis (Variovorax sp. SCN45)
MQIETPPSEKPYEKPEGERRGIVIVNTGDGKGKSTAAFGLALRAHGRGKAVKIYQFMKVP
TARFGEHRMFEQIGIPIEGLGDGFSWKSQDLERSGQLARDGWEKAKAAIMSGEFFLVVLD
EITYPLIYGWLPLDGVLETLRARPRHVHVVLTGRRCPPEIIELADTVTEMTLVKHAFKAG
IPAQRGIED