Protein Info for GFF4438 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF22660: RS_preATP-grasp-like
  • amino acids 1 to 51 (51 residues), 28.5 bits, 2.4e-10
• TIGR01161: phosphoribosylaminoimidazole carboxylase, ATPase subunit
  • amino acids 3 to 329 (327 residues), 371.8 bits, E=2.1e-115
• PF02222: ATP-grasp
  • amino acids 85 to 259 (175 residues), 198.5 bits, E=1.1e-62
• PF17769: PurK_C
  • amino acids 280 to 329 (50 residues), 59.5 bits, 3e-20

Best Hits

Swiss-Prot: 89% identical to PURK_ECOLI: N5-carboxyaminoimidazole ribonucleotide synthase (purK) from Escherichia coli (strain K12)

KEGG orthology group: K01589, 5-(carboxyamino)imidazole ribonucleotide synthase [EC: 6.3.4.18] (inferred from 99% identity to sek:SSPA2036)

MetaCyc: 89% identical to 5-(carboxyamino)imidazole ribonucleotide synthase (Escherichia coli K-12 substr. MG1655)
5-(carboxyamino)imidazole ribonucleotide synthase. [EC: 6.3.4.18]

Predicted SEED Role

"Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)" in subsystem De Novo Purine Biosynthesis (EC 4.1.1.21)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.21

Use Curated BLAST to search for 4.1.1.21 or 6.3.4.18

Protein Sequence (355 amino acids)

>GFF4438 Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPTAVPVQQSVITAEIERWPETALTR
ELARHPAFVNRDVFPIIADRLTQKQLFDKLGLATAPWQLLTSTDEWSGIFDRLGELAIIK
RRVGGYDGRGQWRLRADETGQLPDDCYGECIVERGIHFSGEVSLVGARAHDGSTVFYPLT
HNLHQDGILRTSVAFPQANAEQQEQAESMLSAIMQALNYVGVMAMECFITPEGLLINELA
PRVHNSGHWTQNGASISQFELHLRAITGLPLPAPVINAPSVMINLIGSELNYDWLKLPLV
HLHWYDKAVRPGRKVGHLNLTDSDTSRLSATLEALSPLLPGEYASGIIWAQSKLK