Protein Info for GFF4435 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Conjugative transfer protein TrbB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 TIGR02782: P-type conjugative transfer ATPase TrbB" amino acids 21 to 321 (301 residues), 401.2 bits, see alignment E=1.5e-124 PF00437: T2SSE" amino acids 118 to 295 (178 residues), 113.9 bits, see alignment E=6.9e-37 PF10412: TrwB_AAD_bind" amino acids 147 to 192 (46 residues), 23 bits, see alignment 3.5e-09

Best Hits

Swiss-Prot: 49% identical to TRBB_SINFN: Probable conjugal transfer protein TrbB (trbB) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K03196, type IV secretion system protein VirB11 (inferred from 96% identity to bpt:Bpet2206)

Predicted SEED Role

"Conjugative transfer protein TrbB" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (355 amino acids)

>GFF4435 Conjugative transfer protein TrbB (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSAVAQFPPEPRSSAAASLDRRIQMLRTAMGPLIATALEDPDVVEIMLNPDRTLWIDRLS
SGRAPMGVELSEADGERIIRLVAAHVGAEVHRGQPLLSAELPETGERFEGILPPAAPGPT
FALRKRAIGVIPLERYVVDGMMTAAQAGLLVRAVRERQNVLIAGATSSGKTTLANALLAE
IAATGDRVLVLEDTVELQCAARDHVPLRTRQGVVSMTELVRSSMRLRPDRVVVGEVRGAE
ALDLIKVWGTGHPGGIATIHAGSALGALLRLEQLILEVAVNPPRALIAEAVNVVIHIAGR
GRKRRIESIARVVGFDGVGYRLVDGLADALDAPFPELPSQSDLSSPSLNHPGELP