Protein Info for GFF4434 in Variovorax sp. SCN45

Annotation: Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 971 PF03477: ATP-cone" amino acids 38 to 134 (97 residues), 56.7 bits, see alignment E=4.2e-19 PF00317: Ribonuc_red_lgN" amino acids 292 to 363 (72 residues), 85.4 bits, see alignment 3.5e-28 TIGR02506: ribonucleoside-diphosphate reductase, alpha subunit" amino acids 295 to 907 (613 residues), 716.8 bits, see alignment E=1.1e-219 PF02867: Ribonuc_red_lgC" amino acids 369 to 904 (536 residues), 641.5 bits, see alignment E=1.7e-196

Best Hits

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 84% identity to aav:Aave_0864)

Predicted SEED Role

"Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (971 amino acids)

>GFF4434 Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1) (Variovorax sp. SCN45)
MQTALNPQSTPRAVPSTPPQQQRDTAGSPAGQNLAHYQIIRRNGAVVPFEPNKIAIAMMK
AFLAVHGTQGAASASVRETVDVLTQGVIRALVRSRPGGGTFHIEDVQDQVELGLMRGGHH
EIARAYVLYRERRTQERSKQGEQEAPATPALHVLDNGELVALDINELKGLIESACENLGE
SITAAPIVAETMRNLYDGVPLDEVYKASILAARTLIEKDPDYTFATARLLLHTIFKEIIG
REVMPVDRATAYADYFPQFIKKGVDNDLLDEKLLQYDLPRLGAALKAERDNQFDYLGLQT
LYDRYFLHVRKTRIELPQAFFMRVAMGLSLNEIDREARAIEFYEVLSSFDFMSSTPTLFN
AGTLRSQLSSCYLTTVPDDLDGIYESIKENALLSKFAGGLGNDWTRVRALGSHIKGTNGE
SQGVVPFLKVVNDTAVAVNQGGKRKGAVCTYLETWHLDIEEFLELRKNTGDDRRRTHDMN
TANWIPDLFMRRVMEKGTWTLFSPSNVPDLHDLFGAEFEKAYVAYEEKAARGDIKPARTI
QATDLWRKMLTMLFETGHPWITFKDACNVRSPQQHAGVVHSSNLCTEITLNTSDTETAVC
NLGSVNLLQHLKDGKVDQEKLKKTISTAMRMLDNVIDINYYAVKKARDSNLRHRPVGLGL
MGFQDALYELRIPYASQEAVQFADESMEAICYHAYWASTELAKERGKYSSYKGSLWDKGI
LPIDSLDLLEKARGGYVEVDRSATLDWDVLRQKIKADGMRNSNCVAIAPTATISNIIGVD
ASIEPCFGNLSVKSNLSGEFTVINHYLVRDLKRLGLWDDVMVMDLKHFDGSLRPIDRVPQ
DVKALYATAFEVETTWLVEAAARRQKWIDQAQSLNIYMAGASGKKLDDTYKLAWLRGLKT
TYYLRTQSATHAEKSTVQSGRLNAVSSGGDAPSGMSALEAAAAAAKAQMNAIPATDIAFC
GVDDPTCEACQ