Protein Info for GFF4420 in Variovorax sp. SCN45

Annotation: Conjugative transfer protein TrbL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 459 transmembrane" amino acids 29 to 48 (20 residues), see Phobius details amino acids 68 to 89 (22 residues), see Phobius details amino acids 146 to 169 (24 residues), see Phobius details amino acids 176 to 192 (17 residues), see Phobius details amino acids 207 to 228 (22 residues), see Phobius details amino acids 240 to 261 (22 residues), see Phobius details amino acids 276 to 303 (28 residues), see Phobius details TIGR02783: P-type conjugative transfer protein TrbL" amino acids 1 to 305 (305 residues), 224.1 bits, see alignment E=1.3e-70 PF04610: TrbL" amino acids 37 to 256 (220 residues), 174 bits, see alignment E=2e-55

Best Hits

KEGG orthology group: K07344, type IV secretion system protein TrbL (inferred from 95% identity to bpt:Bpet2212)

Predicted SEED Role

"Conjugative transfer protein TrbL" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (459 amino acids)

>GFF4420 Conjugative transfer protein TrbL (Variovorax sp. SCN45)
MNDVTIIDQFLNTFATYIDSGFGLLRGEVAFLSATLIVIDMTIAGLYWAMSHATGQGDDV
IAKLLRKVLYVGAFAYILNNFNWLASIVFRSFAGLGITATGSAITMENFLQPGRLAKTGI
DAGAPILEQIGEMAGFPEVFVNLDPIVVMFLAWLIVLLCFFVLAIQLFITLIEFKLTTLA
GFVLVPFALWNKTSFLAEKVLGNVVSAGVKVLVLAVIVGIGSGLFAQFQVHPAEPSIDHA
LVIMLASLTLLALGIFGPGIATGLVSGAPQLGAGAMAGAAVGAAGTAVAVGAAATGVGGA
VAAGARMAPAAAKLAGSGARAATSAAGSAKSAFQAGSTAAGGGAKGAMAGLGNVAKTGAQ
SAGRAAASRASAAGQRMAAPFREGWNGDAGASAGQAATGAAPAGAAGAAAAQPPEQPAWA
KRLNRRQQLTHAATTTAHTLRGGDGGGSGQGPSLRDSDS