Protein Info for GFF4419 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Selenophosphate-dependent tRNA 2-selenouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 364 PF00581: Rhodanese" amino acids 11 to 128 (118 residues), 22.3 bits, see alignment E=1.4e-08 TIGR03167: tRNA 2-selenouridine synthase" amino acids 17 to 348 (332 residues), 338.8 bits, see alignment E=1.7e-105 PF26341: AAA_SelU" amino acids 143 to 363 (221 residues), 290.9 bits, see alignment E=6.9e-91

Best Hits

Swiss-Prot: 100% identical to SELU_SALTY: tRNA 2-selenouridine/geranyl-2-thiouridine synthase (selU) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K06917, tRNA 2-selenouridine synthase [EC: 2.9.1.-] (inferred from 99% identity to spq:SPAB_03051)

MetaCyc: 78% identical to tRNA 2-selenouridine synthase (Escherichia coli K-12 substr. MG1655)
RXN0-2281 [EC: 2.9.1.3]

Predicted SEED Role

"Selenophosphate-dependent tRNA 2-selenouridine synthase" in subsystem Selenocysteine metabolism

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.9.1.- or 2.9.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (364 amino acids)

>GFF4419 Selenophosphate-dependent tRNA 2-selenouridine synthase (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MQDRQKAQDYRALLLADTPLIDVRAPIEFEQGAMPGAINLPLMMDDERAAVGTCYKRQGA
DAALALGHRLVCGDIRQQRLEAWKAAYQRFPNGYLCCARGGQRSHIVQRWLQETGIDCPL
IEGGYKALRQTAIQATWQLAQKPILLIGGCTGSGKTQLVRQQPNGVDLEGLARHRGSSFG
RTLNPQLSQASFENKLAVELLKINARQTLKRWVLEDEGRTIGANHLPECLRERMAQAPIA
VVEDPFALRLERLREEYFIRMHHDFTHAYGDEAGWQAYSEYLHHGLFAIRRRLGLQRFAE
LTDTLDRALAEQLSSGSTDGHMAWLVPLLNEYYDPMYRYQLEKKAANIVFRGTWQDVANW
LKAQ