Protein Info for GFF4404 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 833 transmembrane" amino acids 186 to 204 (19 residues), see Phobius details amino acids 216 to 237 (22 residues), see Phobius details amino acids 244 to 266 (23 residues), see Phobius details amino acids 283 to 301 (19 residues), see Phobius details amino acids 435 to 457 (23 residues), see Phobius details amino acids 463 to 485 (23 residues), see Phobius details amino acids 777 to 796 (20 residues), see Phobius details amino acids 802 to 823 (22 residues), see Phobius details PF00403: HMA" amino acids 8 to 61 (54 residues), 35.2 bits, see alignment 2.6e-12 amino acids 104 to 158 (55 residues), 58.3 bits, see alignment 1.6e-19 TIGR01511: copper-translocating P-type ATPase" amino acids 232 to 826 (595 residues), 638.4 bits, see alignment E=2.4e-195 TIGR01525: heavy metal translocating P-type ATPase" amino acids 251 to 826 (576 residues), 605.6 bits, see alignment E=2.3e-185 TIGR01494: HAD ATPase, P-type, family IC" amino acids 290 to 794 (505 residues), 300.1 bits, see alignment E=4.6e-93 PF00122: E1-E2_ATPase" amino acids 319 to 418 (100 residues), 95.8 bits, see alignment E=2.5e-31 PF00702: Hydrolase" amino acids 517 to 731 (215 residues), 133.9 bits, see alignment E=1.9e-42 PF08282: Hydrolase_3" amino acids 704 to 763 (60 residues), 25.6 bits, see alignment 1.9e-09

Best Hits

Swiss-Prot: 100% identical to COPA_SALTY: Copper-exporting P-type ATPase (copA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 94% identity to cko:CKO_02665)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (833 amino acids)

>GFF4404 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVELADVTVTEAHVTGTASADALIETIKQAG
YGATLSHPKAKPLTESSIPSEALAAVPHELPVATADEESQQLLLSGMSCASCVTRVQHAL
QSVPGVTQARVNLAERTALVMGSASAADLVQAVEKAGYGAEAIEDDIKRRERQQETAIAT
MKRFRWQAIVALAVGIPVMVWGMIGDNMMVTDDNRSLWLAIGLITLAVMVFAGGHFYRNA
WKSLLNGTATMDTLVALGTGVAWLYSMSVNLWPQWFPMEARHLYYEASAMIIGLINLGHM
LEARARQRSSKALEKLLDLTPPTARVVTEDGEKSVPLADVQPGMLLRLTTGDRVPVDGEI
TQGEAWLDEAMLTGEPIPQQKGEGDSVHAGTVVQDGSVLFRASAVGSHTTLSRIIRMVRQ
AQSSKPEIGQLADKISAVFVPVVVAIALFSAAIWYFFGPAPQIVYTLVIATTVLIIACPC
ALGLATPMSIISGVGRAAEFGVLVRDADALQRASTLDTLVFDKTGTLTEGKPQVVAIKTF
NGVEEAQALRLAAALEQGSSHPLAHAILEKAGDDKLPQVNGFRTLRGLGVSGEAEGHQLL
LGNQALLNEQHVATDDMTAEITAQASQGSTPVLLAIDGKAAALLAVRDPLRSDSIAALER
LHNAGYRLVMLTGDNPTTANAIAKEAGIDEVIAGVLPDGKADAIKRLQSQGRQVAMVGDG
INDAPALAQADVGIAMGGGSDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGA
FIYNSIGIPVAAGILWPFTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKA