Protein Info for GFF439 in Methylophilus sp. DMC18

Annotation: DNA mismatch repair protein MutS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 848 transmembrane" amino acids 625 to 639 (15 residues), see Phobius details TIGR01070: DNA mismatch repair protein MutS" amino acids 6 to 846 (841 residues), 1107.2 bits, see alignment E=0 PF01624: MutS_I" amino acids 7 to 118 (112 residues), 145.5 bits, see alignment E=1.9e-46 PF05188: MutS_II" amino acids 127 to 250 (124 residues), 83.6 bits, see alignment E=4.2e-27 PF05192: MutS_III" amino acids 267 to 556 (290 residues), 164.8 bits, see alignment E=8.1e-52 PF05190: MutS_IV" amino acids 425 to 516 (92 residues), 96.4 bits, see alignment E=2.4e-31 PF00488: MutS_V" amino acids 607 to 793 (187 residues), 283.5 bits, see alignment E=2.4e-88

Best Hits

Swiss-Prot: 61% identical to MUTS_CHRVO: DNA mismatch repair protein MutS (mutS) from Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)

KEGG orthology group: K03555, DNA mismatch repair protein MutS (inferred from 74% identity to mmb:Mmol_1658)

Predicted SEED Role

"DNA mismatch repair protein MutS" in subsystem DNA repair, bacterial MutL-MutS system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (848 amino acids)

>GFF439 DNA mismatch repair protein MutS (Methylophilus sp. DMC18)
MSFENHTPMMRQYLTLKAQYPDMLLFYRMGDFYELFFEDAEKAARLLGITLTRRGASNGE
PIKMAGLPYHAAEQYLAKLAKVGEAVAICEQVGDPATSKGPVAREVTRILTPGTLTDAAL
LDETRDNTLLCVARGEGVLGLARLNLASGEFVLTEIAPGHLPQELERIHPAELVMAEDHN
AEVLQHARCAKKRLAPWQFDLETASRSLTQQFNTHDLAGFGCGEMPAAVTAAGALLEYVK
HTQRTALPHIVGLRVEQSHHYLQLDAATRRNLEIDTTIRGEASPTLYSLLNSTQTAMGAR
LLRHWLHHPLRNQQTIQQRHLAVAELFEQFLDSALQQALHPVGDIERMTARVALKSARPR
DLSGLRDSLLQLPALVSVLPRTQSGLLQSLIAELQVPAEALQILVQAIQAEPSAVLREGG
VIATGYDAELDDLRAIQTDHGDFLLQFEAAEKARTGITNLKVEYNSVHGFYIEISRLQAE
SAPAEYRRRQTLKNVERFITPELKAFEDKVLSANERALAREKMLYEQVLEALLPHIAQLQ
RIATAVATLDVLACFAERARSLNYVQPQFRADTGIDIQGGRHPVVESIAQPYVANDVVLN
PYRQLLLITGPNMGGKSTFMRQTALIVLLAYCGSFVPAQTAVIGEIDRIFTRIGASDDLA
GGRSTFMVEMTETANILNNATANSLVLLDEIGRGTSTFDGLSLAWAVAKQLLEKNKSLTL
FATHYFELTRIVDEAKHAANVHLDAVEHGQGIVFLHKVEEGAATMSYGIQVAQLAGIPKS
VVQLAKRKLQQLENQTIAATPQNDLFASAVPPEEPPAHPVVAELERIQPDDLTPKQALEL
LYQLKKQL