Protein Info for GFF437 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02430: 3-oxoadipyl-CoA thiolase
  • amino acids 1 to 391 (391 residues), 667.2 bits, E=8.5e-205
• PF00108: Thiolase_N
  • amino acids 1 to 259 (259 residues), 269.5 bits, E=4.5e-84
• TIGR01930: acetyl-CoA C-acyltransferase
  • amino acids 1 to 390 (390 residues), 451.6 bits, E=2.1e-139
• PF00109: ketoacyl-synt
  • amino acids 68 to 112 (45 residues), 23.2 bits, 7.1e-09
• PF02803: Thiolase_C
  • amino acids 268 to 391 (124 residues), 144.6 bits, E=1.8e-46

Best Hits

Swiss-Prot: 67% identical to PCAF_PSEAE: Beta-ketoadipyl-CoA thiolase (pcaF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 71% identity to pmk:MDS_2865)

MetaCyc: 68% identical to beta-ketoadipyl-CoA thiolase (Pseudomonas sp. Y2)
RXN0-6512 [EC: 2.3.1.223]; 3-oxoadipyl-CoA thiolase. [EC: 2.3.1.223, 2.3.1.174]

Predicted SEED Role

"Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)" (EC 2.3.1.-, EC 2.3.1.9)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• phenylacetate degradation I (aerobic) (8/9 steps found)
• adipate degradation (5/5 steps found)
• superpathway of phenylethylamine degradation (9/11 steps found)
• benzoyl-CoA degradation I (aerobic) (6/7 steps found)
• superpathway of salicylate degradation (6/7 steps found)
• ketolysis (3/3 steps found)
• polyhydroxybutanoate biosynthesis (3/3 steps found)
• catechol degradation III (ortho-cleavage pathway) (5/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• 3-oxoadipate degradation (2/2 steps found)
• aromatic compounds degradation via β-ketoadipate (7/9 steps found)
• valproate β-oxidation (7/9 steps found)
• adipate biosynthesis (4/5 steps found)
• ketogenesis (4/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• (2S)-ethylmalonyl-CoA biosynthesis (3/4 steps found)
• acetyl-CoA fermentation to butanoate (5/7 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• acetoacetate degradation (to acetyl CoA) (1/2 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• glycerol degradation to butanol (10/16 steps found)
• 2-deoxy-D-ribose degradation II (4/8 steps found)
• toluene degradation III (aerobic) (via p-cresol) (6/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• 4-methylcatechol degradation (ortho cleavage) (3/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (5/10 steps found)
• isopropanol biosynthesis (engineered) (1/5 steps found)
• pyruvate fermentation to acetone (1/5 steps found)
• pyruvate fermentation to butanol I (3/8 steps found)
• mevalonate pathway I (eukaryotes and bacteria) (2/7 steps found)
• mevalonate pathway II (haloarchaea) (2/7 steps found)
• L-glutamate degradation VII (to butanoate) (5/12 steps found)
• 2-methylpropene degradation (2/8 steps found)
• isoprene biosynthesis II (engineered) (2/8 steps found)
• mevalonate pathway III (Thermoplasma) (2/8 steps found)
• mevalonate pathway IV (archaea) (2/8 steps found)
• L-tryptophan degradation III (eukaryotic) (7/15 steps found)
• ethylmalonyl-CoA pathway (4/11 steps found)
• mandelate degradation to acetyl-CoA (9/18 steps found)
• L-lysine fermentation to acetate and butanoate (3/10 steps found)
• 4-oxopentanoate degradation (2/9 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (7/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (4/13 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (6/17 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (5/16 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (17/35 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)
• superpathway of aerobic toluene degradation (10/30 steps found)
• superpathway of ergosterol biosynthesis I (6/26 steps found)
• superpathway of L-lysine degradation (12/43 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
• superpathway of cholesterol biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Anthocyanin biosynthesis
• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of terpenoids and steroids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Carotenoid biosynthesis - General
• Diterpenoid biosynthesis
• Ether lipid metabolism
• Ethylbenzene degradation
• Fatty acid biosynthesis
• Fatty acid metabolism
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Histidine metabolism
• Limonene and pinene degradation
• Lipopolysaccharide biosynthesis
• Lysine degradation
• Phenylalanine metabolism
• Propanoate metabolism
• Pyruvate metabolism
• Synthesis and degradation of ketone bodies
• Terpenoid biosynthesis
• Tryptophan metabolism
• Tyrosine metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-, 2.3.1.174, 2.3.1.9

Use Curated BLAST to search for 2.3.1.- or 2.3.1.174 or 2.3.1.223 or 2.3.1.9

Protein Sequence (396 amino acids)

>GFF437 Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VRTPIGRYGGALSAIRTDDLGALPLQALMARHPGLDWDQLDEVVYGCVNQAGEDNRNVAR
MSALLAGLPLTAAAVTVNRLCGSGLEAVAGAARGLASGQMQLAIAGGVESMSRAPLVMPK
PTAAFSRHAEVHDSTIGWRFVNPRMETLYGVDSMGDTAENVAEEHGISRADQDAFALRSQ
QRAAAAQQSGVLTQEIVPVSVPGRKGAATVVSVDEHPRADTTAESLAALKPAFRKGGTVT
AGNASGVNDGAAALLMASEAAAQRHGLRPIARIVGAASAGIAPRVMGFGPVPAVHKLLAQ
TGLRMRDFDVIELNEAFAAQGLAVTRALGLPDDAPHVNPNGGAIAFGHPLGMSGARLVLT
AARQLQRTGGRYGLVTMCVGVGQGVALAIERGGDTA