Protein Info for PS417_22360 in Pseudomonas simiae WCS417

Updated annotation (from data): Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2)
Rationale: Specifically important for utilizing Glycine. Automated validation from mutant phenotype: the predicted function (1.4.4.2) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: glycine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 946 transmembrane" amino acids 744 to 759 (16 residues), see Phobius details PF02347: GDC-P" amino acids 14 to 438 (425 residues), 609.7 bits, see alignment E=2.9e-187 amino acids 451 to 729 (279 residues), 42.7 bits, see alignment E=3.7e-15 TIGR00461: glycine dehydrogenase" amino acids 14 to 939 (926 residues), 1495.2 bits, see alignment E=0 PF21478: GcvP2_C" amino acids 768 to 889 (122 residues), 206.8 bits, see alignment E=7.2e-66

Best Hits

Swiss-Prot: 94% identical to GCSP1_PSEPF: Glycine dehydrogenase (decarboxylating) 1 (gcvP1) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 61% identity to apt:APA01_24220)

MetaCyc: 61% identical to glycine cleavage system P-protein (Synechocystis sp. PCC 6803)
GCVMULTI-RXN [EC: 1.4.1.27]

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.27 or 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UBA2 at UniProt or InterPro

Protein Sequence (946 amino acids)

>PS417_22360 Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2) (Pseudomonas simiae WCS417)
MTVQLTTANEFIARHIGPRQEDEQQMLASLGFDSLEALSASVIPESIKGTSVLGLEDGLS
EAEALAKIKAIAGKNQLFKTYIGQGYYNCHTPSPILRNLLENPAWYTAYTPYQPEISQGR
LEALLNFQTLISDLTGLPIANASLLDEATAAAEAMTFCKRLSKNKGSNAFFASIHSHPQT
LDVLRTRAEPLGIDVVVGDERELTDVSPFFGALLQYPASNGDVFDYRELTERFHAAHGLV
AVAADLLALTLLTPPGEFGADVAIGSAQRFGVPLGFGGPHAAYFSTKDAFKRDMPGRLVG
VSVDRFGKPALRLAMQTREQHIRREKATSNICTAQVLLANIASMYAVYHGPKGLTQIARR
VHQLTAILAKGLTALGQNVEQAHFFDTLTLNTGANTAAVHDKARAQRINLRVVDAERVGV
SVDETTTQADIETLWAIFADGKALPDFAAQVESTLPAALLRQSPVLSHPVFNRYHSETEL
MRYLRKLADKDLALDRTMIPLGSCTMKLNAASEMIPVTWAEFGALHPFAPAEQSAGYLEL
TSDLEAMLCAATGYDAISLQPNAGSQGEYAGLLAIRAYHQSRGDERRDICLIPSSAHGTN
PATANMAGMRVVVTACDARGNVDIEDLRAKAIEHRDHLAALMITYPSTHGVFEEGIREIC
GIIHDNGGQVYIDGANMNAMVGLCAPGKFGGDVSHLNLHKTFCIPHGGGGPGVGPIGVKS
HLTPFLPGHAAMERKEGAVCAAPFGSASILPITWMYISMMGGAGLKRASQLAILNANYIS
RRLEEHYPVLYTGSNGLVAHECILDLRPLKDSSGISVDDVAKRLIDFGFHAPTMSFPVAG
TLMIEPTESESKEELDRFCNAMIAIREEIRAVENGTLDKDDNPLKNAPHTAAELVSEWTH
PYTREQAVYPVPSLIEGKYWPPVGRVDNVFGDRNLVCACPSIESYA