Protein Info for GFF4365 in Variovorax sp. SCN45

Annotation: 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00694: Aconitase_C
  • amino acids 90 to 139 (50 residues), 57.9 bits, 1.5e-19
• PF00330: Aconitase
  • amino acids 325 to 517 (193 residues), 195.6 bits, E=1.7e-61
  • amino acids 518 to 645 (128 residues), 98.3 bits, E=5.5e-32

Best Hits

KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 77% identity to adn:Alide_0389)

Predicted SEED Role

"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (5/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35

Protein Sequence (668 amino acids)

>GFF4365 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) (Variovorax sp. SCN45)
MPASTPAIHFCDRILYLGQAPQVVSTVLAGGRVTLTDAAPLRDDVSTDEITPLPILTHFD
EKLARYSYTGVKIAGVLPIGVDAVRASGIEVVVAGKRYGKGSSREHSPAAEKLAGIRLII
AESFERIYRQNADNIGLITSTDFGLIPRIEAGEAIGLDELVAGRDALAAAILKSGGLLRF
GQAQLRSVTPAESVAADRPRTLFEKIVGRHGLATPVTPAAPVVGDGAFVRADWRFIHEYY
TGMAAHMLHGTFGRPLEIREPATVIVFEDHTSYVQESPAHVRGGLVPNVQRMVEAQRAFA
AGYGLRTHRTLTEAEAALDDGSNVAGISHAMMTEHYALPGQLVVGTDSHTPHSGALGCVA
FGVGTTDMANAFVTGAVRMTVPPSLRIVLDGALPPGVTAKDIALNLLARPAIRAGSGVGK
VFEFTGPVVEKLSTDERATLTNMTAELGGFTGIVAPDAETVRFLRERRGVEFVLEDWMRS
DPDAVYAETIHLDCSTVGAMVASPGDPGNGLPLSQVKESVRIDIAYGGSCTAGKREDFDH
YHEVLRWAADRGMRVAPGVELFLQFGTTAVRDQCVAAGYLDAFERVGARILQPSCGACGN
CGPGGSARAEQVTVSAINRNFPGRGGPGSVWLASPPTVAASAIAGELLSFEALKRRHLIQ
KNQNGDKP