Protein Info for GFF4362 in Xanthobacter sp. DMC5

Annotation: Bifunctional enzyme CysN/CysC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 30 to 219 (190 residues), 147.4 bits, E=1.3e-46
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 32 to 438 (407 residues), 664.1 bits, E=1.3e-203
• PF03144: GTP_EFTU_D2
  • amino acids 269 to 329 (61 residues), 35 bits, 5.4e-12
• PF22594: GTP-eEF1A_C
  • amino acids 340 to 439 (100 residues), 105 bits, E=8.8e-34
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 450 to 633 (184 residues), 252.9 bits, E=2.5e-79
• PF06414: Zeta_toxin
  • amino acids 462 to 509 (48 residues), 23.4 bits, 1.3e-08
• PF01583: APS_kinase
  • amino acids 468 to 617 (150 residues), 229.3 bits, E=7.3e-72

Best Hits

Swiss-Prot: 62% identical to NODQ_RHIS3: Bifunctional enzyme NodQ (nodQ) from Rhizobium sp. (strain N33)

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 84% identity to sch:Sphch_3380)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• sulfite oxidation III (2/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (3/6 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (2/5 steps found)
• selenate reduction (2/5 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (2/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (3/12 steps found)
• superpathway of seleno-compound metabolism (5/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Protein Sequence (636 amino acids)

>GFF4362 Bifunctional enzyme CysN/CysC (Xanthobacter sp. DMC5)
MSAPLDPVYKTDALIAEDIDKYLDVHQHKTMLRFITCGSVDDGKSTLIGRLLYDSKMIFE
DQLAALEADSKKVGTQGQELDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPG
HEQYTRNMVTGASTADLAVILVDARKGVLTQTRRHSYLAHLIGIRNIVLAVNKMDLVGYN
REVFEKIVADYRAFATSIGITDFVALPISGFKGDNITTLSPNTPWHAGPTLMEHLETVEI
DAAHDAAKPFRMAVQWVNRPNLDFRGFAGLISTGTVKPGDAIRVLPSGKTSTVSRIVTLD
GDLKEAVAGQSVTLCLADEIDCSRGDVIAAADAPVQAADQFEAVIVWMDEQPMLPGRPYW
LKLGTQTVSATVTEPKYQVNVNNMDHLAAKTLELNAIGSCNIATDRRIPFEPYRENRELG
GFILIDKMTNATVGAGMIHFALRRSDNVHWQATDISREHHAALKNQKPAVVWLTGLSGAG
KSTIANLVEKKLARMNRHTFLLDGDNVRHGLNKDLGFTAADRVENIRRVGEVAKLMTDAG
LIVITAFISPFRAERDMVRGMMQPGEFFEVFIDTPLAEAERRDVKGLYKKARAGQLKNFT
GIDSPYEAPAAPELRVDTTAMDAEEAAEAIVRMLVP