Protein Info for GFF4361 in Variovorax sp. SCN45

Annotation: Gamma-glutamyltranspeptidase (EC 2.3.2.2) @ Glutathione hydrolase (EC 3.4.19.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00066: gamma-glutamyltransferase
  • amino acids 6 to 528 (523 residues), 377.7 bits, E=4.1e-117
• PF01019: G_glu_transpept
  • amino acids 20 to 532 (513 residues), 423 bits, E=1e-130

Best Hits

KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 94% identity to vap:Vapar_1640)

Predicted SEED Role

"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)

MetaCyc Pathways

• γ-glutamyl cycle (4/6 steps found)
• glutathione degradation (DUG pathway) (1/2 steps found)
• leukotriene biosynthesis (1/6 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• peptido-conjugates in tissue regeneration biosynthesis (5/17 steps found)

KEGG Metabolic Maps

• Arachidonic acid metabolism
• Cyanoamino acid metabolism
• Glutathione metabolism
• Selenoamino acid metabolism
• Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.2.2

Use Curated BLAST to search for 2.3.2.2

Protein Sequence (543 amino acids)

>GFF4361 Gamma-glutamyltranspeptidase (EC 2.3.2.2) @ Glutathione hydrolase (EC 3.4.19.13) (Variovorax sp. SCN45)
MKKEAMVVCPQPEAAESGIEILRAGGNAVDAAVATALAQTVVDPLMCGIAGFGTAAVYLP
GTNVHEYFDFHSPAPLAAREDMWEHLLEGETKDGFGFILKGRVNDIGAQSIGTPATLKGL
EAMHQAHGRLPWQQVVEPAIRWATDGYFVRPGMHAFWIDEPTMGRVGNLERLQYCEDSRQ
LYCRPDGRPKPIGAPLRNEGMAAVLRQIAEEGAQPFYEGDLAKKMVAHLQSLGALLTLDD
LARYRVQRNPPLVGRYRDRTVTTNQPPGGGAMLLEMLNILEHFDLAHLEHNSPAYLQRVC
EAMKKATIDKDRCIGDPKFFDVPLDQLLSKDMAREVAEQIRTGHRFNVERVNPGAPVPRD
TTHLSVVDADGNAVSLTHSLAMPSGIITPGMGFLYNGCMGVFDPRPGRAGSIRPGKSRFT
SSCPTMTFRDGELDMVLGAPGGTQIAMGVLQVLLNVIDHGMEMQAAVSAPRFSSTNNSID
VCNRIPRSTTRALEAQGYAIGRNPYNYTIGWVHGVQVQADGLHGGADPGRDGVAYRLRMD
AGH