Protein Info for GFF4359 in Pseudomonas sp. DMC3

Annotation: Queuine tRNA-ribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 TIGR00449: tRNA-guanine family transglycosylase" amino acids 9 to 370 (362 residues), 521.4 bits, see alignment E=1.2e-160 TIGR00430: tRNA-guanine transglycosylase" amino acids 9 to 371 (363 residues), 547 bits, see alignment E=2e-168 PF01702: TGT" amino acids 17 to 369 (353 residues), 540.6 bits, see alignment E=8.8e-167

Best Hits

Swiss-Prot: 95% identical to TGT_PSESM: Queuine tRNA-ribosyltransferase (tgt) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00773, queuine tRNA-ribosyltransferase [EC: 2.4.2.29] (inferred from 98% identity to pfo:Pfl01_4621)

MetaCyc: 70% identical to tRNA-guanine transglycosylase (Escherichia coli K-12 substr. MG1655)
tRNA-guanine transglycosylase. [EC: 2.4.2.29]

Predicted SEED Role

"tRNA-guanine transglycosylase (EC 2.4.2.29)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.29)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (377 amino acids)

>GFF4359 Queuine tRNA-ribosyltransferase (Pseudomonas sp. DMC3)
MSRECRMSFELLATDGKARRGRLTFPRGTVETPAFMPVGTYGTVKGMLPRDIVATGAEII
LGNTFHLWLRPGTQVIKEHGDLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASP
VDGAKVFMGPEESMQVQRDLGSDIVMIFDECTPYPADEDVARVSMELSLRWAQRSKNAHG
DNTAALFGIVQGGMHENLRMRSLEGLDKIGFDGLAIGGLSVGEPKHEMIKVLDYLPGQMP
ADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFIDTGVLKIRNAFHRHDES
PLDPTCDCYTCQNFSRAYLHHLDKCGEMLGSMLNTIHNLRHYQVLMAGLREAIQQGTLAA
FVDAFYAKRGLPVPPLD