Protein Info for GFF4346 in Pseudomonas sp. DMC3

Annotation: Chaperone protein HscA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 621 TIGR01991: Fe-S protein assembly chaperone HscA" amino acids 21 to 617 (597 residues), 909.2 bits, see alignment E=5.8e-278 PF00012: HSP70" amino acids 21 to 601 (581 residues), 612 bits, see alignment E=9.7e-188 PF06723: MreB_Mbl" amino acids 127 to 377 (251 residues), 51.8 bits, see alignment E=6e-18

Best Hits

Swiss-Prot: 97% identical to HSCA_PSEPF: Chaperone protein HscA homolog (hscA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K04044, molecular chaperone HscA (inferred from 97% identity to pfo:Pfl01_4608)

MetaCyc: 66% identical to iron-sulfur cluster biosynthesis chaperone HscA (Escherichia coli K-12 substr. MG1655)
3.6.4.-

Predicted SEED Role

"Chaperone protein HscA" in subsystem Protein chaperones

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (621 amino acids)

>GFF4346 Chaperone protein HscA (Pseudomonas sp. DMC3)
MALLQIAEPGQSPQPHQRRLAVGIDLGTTNSLVAALRSGLSEPLADANGQVILPSAVRYH
ADRVEVGESAKLAAASDPLNTVLSVKRLMGRGLSDVKQLGEQLPYRFVGGESHMPFIDTV
QGPKSPVEVSADILKVLRQRAEATLGGELVGAVITVPAYFDDAQRQATKDAAKLAGLNVL
RLLNEPTAAAVAYGLDQHAEGLVAIYDLGGGTFDISILRLTGGVFEVLATGGDSALGGDD
FDHAIAGWIIESAGLSADLDPGAQRNLLQTACAAKEALTDAASVEVAYGDWKAQLTREAF
DALIEPMVARSLKACRRAVRDSGIELDDVHAVVMVGGSTRVPRVREAVAEAFGRQPLTEI
DPDQVVAIGAAIQADTLAGNKRDGGELLLLDVIPLSLGLETMGGLMEKVIPRNTTIPVAR
AQDFTTYKDGQSAMAIHVLQGERELISDCRSLARFELRGIPAMVAGAAKIRVTFQVDADG
LLSVSARELGSGVEASIQVKPSYGLTDGEIAKMLKDSFQHANDDKVARVLREQQVDAQRL
LEAVQGALEADGERLLDAEERMVIELQMQELAELMKGTDGYAIEQQTKRLSQVTDAFAAR
RMDLTVKAALSGRNLNEIEDI