Protein Info for GFF4344 in Xanthobacter sp. DMC5

Annotation: Bicyclomycin resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 22 to 42 (21 residues), see Phobius details amino acids 55 to 76 (22 residues), see Phobius details amino acids 88 to 110 (23 residues), see Phobius details amino acids 116 to 134 (19 residues), see Phobius details amino acids 146 to 168 (23 residues), see Phobius details amino acids 175 to 195 (21 residues), see Phobius details amino acids 227 to 250 (24 residues), see Phobius details amino acids 262 to 280 (19 residues), see Phobius details amino acids 292 to 310 (19 residues), see Phobius details amino acids 317 to 338 (22 residues), see Phobius details amino acids 348 to 370 (23 residues), see Phobius details amino acids 380 to 401 (22 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 27 to 395 (369 residues), 243.5 bits, see alignment E=2.6e-76 PF07690: MFS_1" amino acids 31 to 369 (339 residues), 150.2 bits, see alignment E=3.8e-48

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 76% identity to xau:Xaut_1990)

Predicted SEED Role

"Multidrug resistance transporter, Bcr/CflA family" in subsystem Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>GFF4344 Bicyclomycin resistance protein (Xanthobacter sp. DMC5)
VTATDPAEAPQQRAPLMTEREVSIIGGMMVMLGPVSLSLYTPALPTLVEVFGTSIATVKL
TLTVFFFGFAVSQLICGPLSDAYGRRPVALGFFGIYLSGAVVAMLAPTIGWLVAGRILQG
IGAAAGIALSRALVRDQYTGQASARVMNLIGTILAIGPAISPTIGGFLLGAFGWHSVFVA
MTCFGLALMTLLLLRVPETNRTPDPRLAAPRAVAASYRRLLSDRGFMRAALTAGSALGGI
YTLAAALPFVLIDRVGLSPTQFGVAMMMQTGAFMFGTLIARPLLNRFKAQQLILPGLLLV
LTGAIAMAVGPHVVALSTLSVMLPISFWACGCALLLPGCTTNALARFGSIAGAASALTGF
LQIGSGFIGTSISALLPSPLLAMTILVPAMAVMALTIHLALAPRREAAPLEVEPARDLAP
DLELAADPLGLVGASGDEIEAGTYRRAS