Protein Info for GFF4335 in Variovorax sp. SCN45

Annotation: Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.8.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 PF02771: Acyl-CoA_dh_N" amino acids 4 to 114 (111 residues), 101.4 bits, see alignment E=7.8e-33 PF02770: Acyl-CoA_dh_M" amino acids 119 to 215 (97 residues), 84.8 bits, see alignment E=7.5e-28 PF00441: Acyl-CoA_dh_1" amino acids 227 to 378 (152 residues), 173.9 bits, see alignment E=5.2e-55 PF08028: Acyl-CoA_dh_2" amino acids 244 to 366 (123 residues), 87.9 bits, see alignment E=1.4e-28

Best Hits

Swiss-Prot: 46% identical to ACDP_MYCTO: Probable acyl-CoA dehydrogenase fadE25 (fadE25) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: None (inferred from 49% identity to sgr:SGR_4482)

MetaCyc: 50% identical to 5-carboxy-2-pentenoyl-CoA reductase (Thermobifida fusca B6)
RXN-22975

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2

Use Curated BLAST to search for 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (384 amino acids)

>GFF4335 Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.8.1) (Variovorax sp. SCN45)
MDFTPEEIAFRDSVRRMAERHVAPIAAEIDENDRFPTELIPVYGDIGLLQLWVPEAYGGP
GANLTMVCMAREEISRHSEACGLLAGETTMFVLPLMHFGTEAQKQRFLPIVAQGRTLTAI
ALSEPGAGSDVSAIRTRAVRDGDSYVINGQKQWCSFGSVADYIMVFAKTSDAGGVDNISP
FIVDVKTMPGVSFGKKERKMGMRGAVNVPVYLDNVRVPAENMLGEEGKGFRAAMRALDLN
RPAIAAASVGLAQGALDAAVAYAQQRKQFKRFISEFQGVQFMLADMAMQIQAARCLLYEC
TRAGDRGEWDRLPMLASMAKCFASDMAMKVTTDAVQVLGGYGYTKDYPVERMMRDAKLNQ
IFEGTNQIQRVVIARELLRNAAAA