Protein Info for GFF4320 in Pseudomonas sp. DMC3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 842 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00358: PTS_EIIA_1" amino acids 8 to 129 (122 residues), 136.1 bits, see alignment E=1.4e-43 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 9 to 127 (119 residues), 124.3 bits, see alignment E=4e-40 TIGR01003: phosphocarrier, HPr family" amino acids 173 to 250 (78 residues), 72.3 bits, see alignment E=3.7e-24 PF00381: PTS-HPr" amino acids 173 to 251 (79 residues), 87 bits, see alignment E=1.8e-28 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 276 to 825 (550 residues), 504.6 bits, see alignment E=5e-155 PF05524: PEP-utilisers_N" amino acids 276 to 392 (117 residues), 81.3 bits, see alignment E=1.6e-26 PF00391: PEP-utilizers" amino acids 418 to 490 (73 residues), 66.5 bits, see alignment E=3.2e-22 PF02896: PEP-utilizers_C" amino acids 521 to 802 (282 residues), 341 bits, see alignment E=1.4e-105

Best Hits

KEGG orthology group: K11189, phosphocarrier protein (inferred from 68% identity to pfl:PFL_4931)

Predicted SEED Role

"PTS system, glucose-specific IIA component / Phosphotransferase system, phosphocarrier protein HPr / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (842 amino acids)

>GFF4320 hypothetical protein (Pseudomonas sp. DMC3)
MATPQQLQLLAPLSGVLMALDQVPDPVFSGRIIGDGLCIDPTSSTLCAPMAGVVSDVQAS
GHALTITSDAGVQVLMHIGLDTVNLAGEGFTRRVEAGQRVEAGQALIDFDADYIALHARS
LLTLMLVVSGEPFDSLVGSTGLVEVGQPVLDVVVGAAVGEHPAPVPGDAVFSQPINLPNL
NGLHARPAAVFAQAAKAFSATIHLHKLQAQANGKSLVAIMALQTAHGDSVQVSAEGEDAE
AAVAALTRLLSEGCGEAVTAPAPVEADAAEMPNILRGVCASPGSALGQVMQIVEASLLIN
EFGAEPPVELEALSQALIEADMALQHLRDTASGQAQAEIFKAHQELLADPSLLDQARALI
DAGNSAAFAWKTSTDATAAMFRSLGNALLAERAADLDDVGQRVLKLILGVHEQTLELPDD
TILIADQLTPSQTAGLDTRKVLGFATVGGGATSHVAILARACGLPAICGMPLQMLGLGNG
TRVLLDADKGELQLHPDAESIDQLRTRHERQRQRHQQELAQASEAACTRDGQHIEVTANV
ASQGETAQAMELGASGVGLLRSEFLYLGRQQAPSHDEQVATYSAIARTIGPAHNLVVRTL
DVGGDKPLAYVPMDSETNPFLGLRGIRLCLERPQLLREQFRAILACCELTRLHIMLPMVT
NLAELRLARQILEQEARALGLAHLPKLGIMIEVPAAALMADVLAPHVDFFSIGTNDLTQY
TLAMDRDHPRLASQADSFHPSVLRLIAMTVKAAHAHGKWVGVCGAMASERLAVPLLLGLG
VDELSVSVPLIPAIKSAVREVNMADCRIIAEQVSNLESAADVRAALQQFHGAKVDASPVM
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