Protein Info for GFF429 in Variovorax sp. SCN45

Annotation: RND efflux system, inner membrane transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1089 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 343 to 362 (20 residues), see Phobius details amino acids 369 to 390 (22 residues), see Phobius details amino acids 396 to 419 (24 residues), see Phobius details amino acids 440 to 462 (23 residues), see Phobius details amino acids 472 to 499 (28 residues), see Phobius details amino acids 548 to 567 (20 residues), see Phobius details amino acids 877 to 898 (22 residues), see Phobius details amino acids 905 to 927 (23 residues), see Phobius details amino acids 933 to 956 (24 residues), see Phobius details amino acids 980 to 1000 (21 residues), see Phobius details amino acids 1010 to 1036 (27 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 3 to 1042 (1040 residues), 1215 bits, see alignment E=0 PF00873: ACR_tran" amino acids 4 to 1036 (1033 residues), 1217.2 bits, see alignment E=0 PF03176: MMPL" amino acids 310 to 511 (202 residues), 49.9 bits, see alignment E=3.6e-17 amino acids 846 to 1039 (194 residues), 25.8 bits, see alignment E=7.4e-10 PF02355: SecD_SecF_C" amino acids 342 to 498 (157 residues), 23.7 bits, see alignment E=4.2e-09

Best Hits

KEGG orthology group: None (inferred from 87% identity to aaa:Acav_1398)

Predicted SEED Role

"RND multidrug efflux transporter; Acriflavin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1089 amino acids)

>GFF429 RND efflux system, inner membrane transporter (Variovorax sp. SCN45)
MNLSKFFIDRPIFAGVLSLLMLIAGLIALRGLPISEYPEVAPPSVVVRAQYPGANPKVIA
ETVATPLEEQINGVEGMLYMGSQATTDGVLTLTVTFRLGTDPDKAQQLVQNRVSQAEPRL
PEEVRRLGITTVKSAPDLTMVVHLVSPNNRYDINYLRNYAVLNVKDPLARIEGVGQVQIF
GGGDYSMRVWLDPQKVAQRGLSASDVVAAIRGQNVQAAAGVVGASPGLSGVDMQLSINAQ
GRLQSEEEFGDIIVKSGTDGAVTRLRDIGRLEMGAADYSLRSLLNNDPAVGMGVFQAPGS
NALDISANVRKTMEELNKNMPEGLEYRIAYDPTQFVRASIESVIHTLLEAIMLVVLVVIL
FLQTWRASIIPLLAVPVSVIGTFAVLHVLGFSINALSLFGLVLAIGIVVDDAIVVVENVE
RNIEAGLTPREATYRAMREVSGPIIAIALVLVAVFVPLAFISGLTGQFYRQFAVTIAIST
VISAINSLTLSPALAALLLRGHDQPKDALTRGMDRAFGWLFRGFNKLFHRGSEAYSGGVK
NVISRKALMLAIYVALIAVTFGLFKAVPGGFVPAQDKQYLIGFAQLPDGATLDRTDEVIQ
RMGEIMKKNPNVEDAIAFPGLSINGFTNSSNSGIVFATLKPFDQRKRPDQSGGAVAGQLN
GAFAGIQDAFIVMFPPPPVAGLGTTGGFKLQLEDRASVGYDQMDAAVKAFMAKAQQAPEL
AGMFTSWQVNVPQLYADIDRTKARQLGVPVTDIFDTMQIYLGSLYANDFNKFGRTYSVRV
QADAPYRARAEDVGLLKVRSTSGEMVPLSALMKVNSTFGPERAMRYNGYLAADINGGPAP
GYSSGQAQDAITKIAAETLPKGVSFEWTELTYQEILAGNSAFLVFPLAILLVFLVLAAQY
ESLTLPIAIILIVPMGIMAAMAGVWISGGDNNVFTQIGLIVLVGLSAKNAILIVEFAREL
EFAGRTPVQAAIEASRLRLRPILMTSLAFVMGVLPLVLSTGAGSEMRKAMGVAVFAGMIG
VTAFGLFLTPVFYVLLRRLAGNRPLKLHGEVPHGEDFVSADHPATPSLGGSGGGGLHPMP
ASPRPSHDD