Protein Info for GFF4281 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 2-isopropylmalate synthase (EC 2.3.3.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 513 TIGR00973: 2-isopropylmalate synthase" amino acids 4 to 500 (497 residues), 700.2 bits, see alignment E=6.1e-215 PF00682: HMGL-like" amino acids 5 to 274 (270 residues), 323.1 bits, see alignment E=1.5e-100 PF08502: LeuA_dimer" amino acids 371 to 502 (132 residues), 130.8 bits, see alignment E=3e-42

Best Hits

Swiss-Prot: 88% identical to LEU1_ACISJ: 2-isopropylmalate synthase (leuA) from Acidovorax sp. (strain JS42)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 88% identity to ajs:Ajs_1774)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (513 amino acids)

>GFF4281 2-isopropylmalate synthase (EC 2.3.3.13) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSDKLIIFDTTLRDGEQSPGASMTKDEKLRIARQLERLKVDVIEAGFAASSNGDFDCVQA
IANTIKDSTVCSLARANDRDIARAAEALKGAHRARIHTFIATSALHMEKKLRMTPDQVLE
QAVQSVRFARNLVDDIEFSPEDGYRSDVDFLCRVLEAVIKEGATTLNIPDTVGYAIPELY
GDFIRTLRERVPNSDKAIWSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNC
SLEEIVMAVRTRRDYFGLDVGVDATQIVPASRMVSQTTGFVVQPNKAVVGANAFAHASGI
HQDGVLKARDTYEIMRAEDVGWSANKIVLGKLSGRNAFKQRLQDLGIEMESESEINTAFA
AFKELADRKSEIFDEDILALCSDESVTAEKEQYGLVSLKQRSETGERPHASVVFTVDGKE
IQGESDGNGPVDASLKAIENHVKSGAELLLYSVNAITSGSTESQGEVTVRLQHGGRVVNG
VGADPDIVVASAKAYLSALNKLHSKSDRVAAQG