Protein Info for GFF4280 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 2-isopropylmalate synthase (EC 2.3.3.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 562 TIGR00970: 2-isopropylmalate synthase" amino acids 5 to 551 (547 residues), 788 bits, see alignment E=2.5e-241 PF00682: HMGL-like" amino acids 31 to 311 (281 residues), 244.8 bits, see alignment E=1.2e-76 PF08502: LeuA_dimer" amino acids 420 to 549 (130 residues), 95.9 bits, see alignment E=1.9e-31

Best Hits

Swiss-Prot: 62% identical to LEU1_PSEPK: 2-isopropylmalate synthase (leuA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 79% identity to pol:Bpro_2321)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (562 amino acids)

>GFF4280 2-isopropylmalate synthase (EC 2.3.3.13) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MLKHPASKYPAFQPIGLKDRTWPDAVITQAPIWLSTDLRDGNQALIEPMDVERKLRMFKL
LVQIGFKEIEVGFPSASQTEFDFLRRLIDENLIPDDVTIQVLTQAREPLIRRTFEALQGA
PRAIVHLYNAVAPVMRRVVLGMDEDEIVELATSQARLFSELAASQPATQWTFQYSPEMFS
GTELAFSKRVVDAVTEVWQPTPEHKCIINLPSTVEHSTPNIFADMIEWMHRHLARRDAIV
LSVHPHNDRGTGTAAGEFALMAGADRIEGCLFGNGERTGNLDLVNVALNLYTQGVSPGLD
FSDIDEIRRTVEHCNQLPVHPRHPYVGDLVYTSFSGSHQDAIRKAFAARREDDPWNIPYL
PVDPKDLGRSYEAVIRVNSQSGKGGIAYLLENEYGLELPRRLQIEFSQVVQAVMDANGKE
LAAADLWDIFDREYGLSQVAAPLQPVMEDLTGQNVRVAAQVEMAGQPVSVDGQGNGPIDA
FVNGLQAVMGRQLRVLDYHEHAIGSGAQARAVAYVEMRIDEQRTVFGVGVDANIVSASFK
AILSGLNRVTRPHTNTSEAQTV