Protein Info for GFF4269 in Pseudomonas sp. DMC3

Annotation: p-hydroxybenzoic acid efflux pump subunit AaeB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 680 transmembrane" amino acids 7 to 26 (20 residues), see Phobius details amino acids 32 to 48 (17 residues), see Phobius details amino acids 55 to 74 (20 residues), see Phobius details amino acids 80 to 98 (19 residues), see Phobius details amino acids 106 to 124 (19 residues), see Phobius details amino acids 140 to 156 (17 residues), see Phobius details amino acids 358 to 379 (22 residues), see Phobius details amino acids 385 to 402 (18 residues), see Phobius details amino acids 412 to 431 (20 residues), see Phobius details amino acids 437 to 455 (19 residues), see Phobius details amino acids 461 to 481 (21 residues), see Phobius details amino acids 493 to 513 (21 residues), see Phobius details PF04632: FUSC" amino acids 6 to 661 (656 residues), 551.2 bits, see alignment E=4.2e-169 PF13515: FUSC_2" amino acids 19 to 150 (132 residues), 62.5 bits, see alignment E=4.4e-21

Best Hits

KEGG orthology group: None (inferred from 93% identity to pfo:Pfl01_0171)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (680 amino acids)

>GFF4269 p-hydroxybenzoic acid efflux pump subunit AaeB (Pseudomonas sp. DMC3)
MPPARDWFYGVRTFAASMIALYIALLMQMPRPYWAMATVYIVSSPFLGPTSSKALYRAAG
TFLGAAAAVLFVPMFVQSPYVLVVVIALWTGILLFMSLHLRTANSYALMLAGYTLPLIAL
PVVDNPLAVWDVAEARTEEIFLGIAVAAVVGAMFWPRRLAPVFNDAVSKWFADATSYSLK
FLSRDVQPEEVTALRMAMVANFNSLELMIGQLPHEGARPQTVRNTKELRGRMIHLLPVID
ALEDSLYALERRTPELVEKFQPLLTATREWLAYADANLERWQALRDQLDALQPSAEALEE
RKQLLFSNAIYRLGEFIDLWQDCRSLQDAILCERQDSWRAVYRHWRLGRLTPFIDRGLML
YSVTSTILAIIVASVLWILLGWPDGGSAVILAAVACSFFASMDDPAPQIYRFFFWTGMSV
LFASLYLFLILPNLHDFPMLVLAFAVPFICVGTLTVQPRFYLGMLLTLVNTSSFISIQGA
YDADFFAFANSNLAGPLGLLFAFIWTLVARPFGAELAAKRLTRFSWKDIVSMTEPASLAE
HRHLGVQLLDRLMQHLPRLALTGQDTGIAMREVRVGLNMLDLLAYTPRVTGVPNALLRQV
VGEVGEYFRACLKADERLPAPSGLLMTLDRTRRALNGQGDDETRRHLLHALSGLRLALLP
GVEFVSSAEPEEPLPDGAPL