Protein Info for GFF4261 in Pseudomonas sp. DMC3

Annotation: Poly-beta-1,6-N-acetyl-D-glucosamine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 transmembrane" amino acids 5 to 25 (21 residues), see Phobius details amino acids 33 to 54 (22 residues), see Phobius details amino acids 224 to 241 (18 residues), see Phobius details amino acids 319 to 344 (26 residues), see Phobius details amino acids 364 to 384 (21 residues), see Phobius details amino acids 396 to 419 (24 residues), see Phobius details TIGR03937: poly-beta-1,6 N-acetyl-D-glucosamine synthase" amino acids 30 to 438 (409 residues), 641.3 bits, see alignment E=3.3e-197 PF13641: Glyco_tranf_2_3" amino acids 77 to 299 (223 residues), 104.6 bits, see alignment E=2.6e-33 PF10111: Glyco_tranf_2_2" amino acids 78 to 172 (95 residues), 30.9 bits, see alignment E=6.3e-11 PF00535: Glycos_transf_2" amino acids 78 to 245 (168 residues), 120.5 bits, see alignment E=2.2e-38 PF03142: Chitin_synth_2" amino acids 134 to 257 (124 residues), 28.1 bits, see alignment E=2.6e-10 PF13506: Glyco_transf_21" amino acids 143 to 297 (155 residues), 42.6 bits, see alignment E=1.4e-14 PF13632: Glyco_trans_2_3" amino acids 159 to 408 (250 residues), 86 bits, see alignment E=9.7e-28

Best Hits

KEGG orthology group: K11936, biofilm PGA synthesis N-glycosyltransferase PgaC [EC: 2.4.-.-] (inferred from 96% identity to pfo:Pfl01_0179)

Predicted SEED Role

"Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-)" (EC 2.4.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.-.-

Use Curated BLAST to search for 2.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (451 amino acids)

>GFF4261 Poly-beta-1,6-N-acetyl-D-glucosamine synthase (Pseudomonas sp. DMC3)
MLDRLLALLVLAIVLGVPLGLIFLVTGQFLMSFVFFYPLFMSGLWIAGGLYFWLHWERHW
PWQDDTLPPPLEGEPLISILIPCYNEGDNAADTIYAALAQHYPNIEVIAINDGSKDNTAE
VLDALAKEDPRLRVLHLAENQGKAVALRMGAIAARSEYLVCIDGDALLAPNTAAYLVAPM
LDNARLGAVTGNPRIRTRSTLIGRVQVGEFSSIIGLIKRTQRVFGRIFTVSGVIVAFRRT
ALNRVGYWSPDMITEDIDISWKLQLDHWSIFYEPRALCWILMPETLGGLWKQRLRWAQGG
AEVLFKNIRGIWQYRHRYLWPLLFEYCLSTGWAFTFLLSVIFWGMGKFMVMPDAIAVDHL
MPPAFTGLLLAFVCLVQFAVSIIIDRRYEPGLGKTMFWVVWYPLVFWLVSLLTTLVSFPK
VLFSQHKKRARWVSPDRGIKPLGDDQEEVIK