Protein Info for GFF4257 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Hydroxyacylglutathione hydrolase (EC 3.1.2.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 257 TIGR03413: hydroxyacylglutathione hydrolase" amino acids 4 to 256 (253 residues), 338.2 bits, see alignment E=1.4e-105 PF00753: Lactamase_B" amino acids 14 to 168 (155 residues), 72.5 bits, see alignment E=6.9e-24 PF12706: Lactamase_B_2" amino acids 24 to 151 (128 residues), 31.9 bits, see alignment E=1.5e-11 PF16123: HAGH_C" amino acids 169 to 256 (88 residues), 106.3 bits, see alignment E=1.3e-34

Best Hits

Swiss-Prot: 66% identical to GLO2_RHOFT: Hydroxyacylglutathione hydrolase (gloB) from Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)

KEGG orthology group: K01069, hydroxyacylglutathione hydrolase [EC: 3.1.2.6] (inferred from 67% identity to vei:Veis_0591)

Predicted SEED Role

"Hydroxyacylglutathione hydrolase (EC 3.1.2.6)" in subsystem Glutathione: Non-redox reactions or Methylglyoxal Metabolism (EC 3.1.2.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (257 amino acids)

>GFF4257 Hydroxyacylglutathione hydrolase (EC 3.1.2.6) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MHLIPIPAFSDNYIWALHDGARALVVDPGESAGVARWLKQEGLRLDTILITHHHADHTGG
VVELREATGARVIGPAYEPMPEPLQRVGEGESVEVLGLRFDVIEVPGHTAGHIAYYTPAM
DGAPLLFCGDTLFSGGCGRLFEGTPAQMLDSLTRLAALPSHTRVCCTHEYTLSNLKFAQA
VEPGNAELAAYTARCQAQRAQNFPTLPSSIGLEKQINPFLRTDETAVVQSAQAHEPGIAA
DPVSVFATLREWKNNFR