Protein Info for GFF4251 in Pseudomonas sp. DMC3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 transmembrane" amino acids 12 to 28 (17 residues), see Phobius details amino acids 45 to 64 (20 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 84 to 208 (125 residues), 57.6 bits, see alignment E=1.4e-19 PF13188: PAS_8" amino acids 91 to 128 (38 residues), 29.6 bits, see alignment 1.4e-10 PF00989: PAS" amino acids 94 to 198 (105 residues), 44.5 bits, see alignment E=4.4e-15 PF13426: PAS_9" amino acids 98 to 199 (102 residues), 56.3 bits, see alignment E=1e-18 PF08447: PAS_3" amino acids 109 to 194 (86 residues), 30.5 bits, see alignment E=1.1e-10 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 208 to 371 (164 residues), 134.8 bits, see alignment E=2.4e-43 PF00990: GGDEF" amino acids 212 to 368 (157 residues), 162.7 bits, see alignment E=2e-51 PF00563: EAL" amino acids 390 to 624 (235 residues), 259.5 bits, see alignment E=7.7e-81

Best Hits

KEGG orthology group: None (inferred from 90% identity to pfo:Pfl01_0192)

Predicted SEED Role

"GGDEF domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (639 amino acids)

>GFF4251 hypothetical protein (Pseudomonas sp. DMC3)
MSATYRDALRAALLYLVLAAVWLQVSDHLLSRFFDDSSALARWQLINGYLLAAVSAGLIF
LARARLCRFLGIGARLRERHSDRERLRQAAAVFDCTREGVLVTDHRGLIVHVNRAFIEIT
GYRRDEVIGQQPSLFKSGHHPPGFYQAMFATLEAEGEWSGEIWNRRKTGEIYPQWQTIRV
IHDDFGRLSHYVAVFSDISAIKDSESELKHLAHHDPLTDLPNRLLFSDRAEQALASAQTH
KRGCALLMLDLDHFKMINDSLGHNVGDRLLKAVATRLQALFGPGITLARLGGDEFAVLAE
SCPQPAQAAALAQRTLDALKEPVLVDGNQLFINASIGISLFPSDALSAEQLLRNADSALF
KAKSSGRNGYALYTEELTAHAQQRVEIAFELRRALEQEELRVYYQPVHDLQSSRLVGVEA
LVRWQHPQRGLVSPAEFIPIAERTGLIAEIDAWVMQQACRQMCQWQQSGVVLAFVAVNVS
SRLFARRELYQQVAQVLHDTGLDPAYLELEVTESAVMDDPEVALEQMHRLRELGVRLAID
DFGTGYSSLLRLKRLPVQKLKIDQGFVAGLPWDEDDVAIVRVIIALARSMGMQVHAEGIE
QAEQAAFLLAQECDLGQGYWFGRPVSADRLDWAKTPLIG