Protein Info for GFF4248 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 762 transmembrane" amino acids 99 to 119 (21 residues), see Phobius details amino acids 135 to 154 (20 residues), see Phobius details amino acids 170 to 189 (20 residues), see Phobius details amino acids 201 to 219 (19 residues), see Phobius details amino acids 353 to 375 (23 residues), see Phobius details amino acids 381 to 403 (23 residues), see Phobius details amino acids 697 to 717 (21 residues), see Phobius details amino acids 723 to 744 (22 residues), see Phobius details PF00403: HMA" amino acids 14 to 70 (57 residues), 70.4 bits, see alignment 3.4e-23 TIGR01511: copper-translocating P-type ATPase" amino acids 151 to 747 (597 residues), 597.5 bits, see alignment E=6e-183 TIGR01525: heavy metal translocating P-type ATPase" amino acids 169 to 746 (578 residues), 626.9 bits, see alignment E=7.8e-192 TIGR01494: HAD ATPase, P-type, family IC" amino acids 208 to 716 (509 residues), 310.7 bits, see alignment E=2.9e-96 PF00122: E1-E2_ATPase" amino acids 237 to 417 (181 residues), 197.9 bits, see alignment E=2.7e-62 PF00702: Hydrolase" amino acids 435 to 650 (216 residues), 132.2 bits, see alignment E=7.6e-42 PF08282: Hydrolase_3" amino acids 625 to 683 (59 residues), 21.1 bits, see alignment 5.8e-08

Best Hits

Swiss-Prot: 66% identical to ATCU_SINMW: Copper-transporting P-type ATPase (actP) from Sinorhizobium medicae (strain WSM419)

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to stm:STM0353)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (762 amino acids)

>GFF4248 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSQSENRHDTISLLIEGMTCASCVARVEKGIKAVPGVTDATVNLATERATVRGTASAEAV
IAAIEKTGYKARPIETAGQGEDDSEEKKEAERVRLKRDLILASVLALPVFVLEMGSHLIP
GMHEWVIKTIGLQQSWYWQFALTLLVLTIPGRRFYLKGFPALARLAPDMNSLVAVGTAAA
FGYSLVATFTPDLLPEGTVNVYYEAAAVIVALILLGRFLEARAKGRTSEAIKRLVGLQAR
VAHVLREGRIVDIPVDEVVLGDCVEVRPGERIPVDGEVTEGRSFVDESMITGEPIPVEKS
AGSAVVGGTVNQKGALTLRATAVGGQTMLAQIIRLVEQAQGSKLPIQAVVDKVTLWFVPM
VMLIAALTFVVWLAFGPSPALTFALINGVAVLIIACPCAMGLATPTSIMVGTGRGAEMGV
LFRKGEALQLLKDAKVVAVDKTGTLTEGRPVLTDLDVASGFERREVLAKVAAVESRSEHP
IARAIVVSAEEEGIALPGMSGFESVTGMGVYATVDGTRVDVGADRYMREIGVDISGFATT
AERLGQEGKSPLYAAIDGQLAAIIAVADPIKPSTPAAINALHQLGIKVAMITGDNARTAQ
AIARQLGIDDVVAEVLPEGKVEAIRRLKAAYGQVAFVGDGINDAPALAESDVGLAIGTGT
DVAVESADVVLMSGNLQGVPNAIALSKATIRNIHQNLFWAFAYNTALIPVAAGALFPVWG
ILLSPVFAAGAMAMSSVFVLGNALRLRRFRAPMATPSDTSTT