Protein Info for PS417_21745 in Pseudomonas simiae WCS417

Updated annotation (from data): ABC transporter for L-Arginine, substrate-binding component
Rationale: Specific phenotype on L-Arginine. The ATPase component of this system is not clear. PS417_21725 is nearby and similar to aotP, but has no phenotype on arginine. It might be redundant with another ATPase? (SEED_correct) (KEGG_correct)
Original annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 261 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01096: lysine-arginine-ornithine-binding periplasmic protein" amino acids 3 to 251 (249 residues), 285.1 bits, see alignment E=2.4e-89 PF00497: SBP_bac_3" amino acids 26 to 252 (227 residues), 135 bits, see alignment E=1.4e-43

Best Hits

Swiss-Prot: 44% identical to ARGT_ECOLI: Lysine/arginine/ornithine-binding periplasmic protein (argT) from Escherichia coli (strain K12)

KEGG orthology group: K10022, arginine/ornithine transport system substrate-binding protein (inferred from 92% identity to pfs:PFLU4765)

Predicted SEED Role

"Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein" in subsystem Arginine and Ornithine Degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UDY9 at UniProt or InterPro

Protein Sequence (261 amino acids)

>PS417_21745 ABC transporter for L-Arginine, substrate-binding component (Pseudomonas simiae WCS417)
MKKLVLLGALALSVLSMQAFAEGKPLKIGIEAAYPPFASKAPDGSIVGFDYDIGNALCEE
MKVKCTWVEQEFDGLIPALKVRKIDAILSSMSITDDRKKSVDFTNRYYLTPARLVLKEGT
AVSDSLDELKGKKIGVQRGSIHDRFAKEVLAPKGATIVPYSSQNEIYLDVEAGRLDGTVA
DATLLQEGFLDTPAGKGYAFTGPAFTDAKYFGDGIGIAVRKGDKENLDRINAAIAAIRAN
GKYKEIEKKYFNFDIYGPEAQ