Protein Info for GFF4233 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 266 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details TIGR00972: phosphate ABC transporter, ATP-binding protein" amino acids 20 to 266 (247 residues), 397.4 bits, see alignment E=1.1e-123 PF00005: ABC_tran" amino acids 35 to 191 (157 residues), 124.9 bits, see alignment E=3.9e-40

Best Hits

Swiss-Prot: 77% identical to PSTB_BORPE: Phosphate import ATP-binding protein PstB (pstB) from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

KEGG orthology group: K02036, phosphate transport system ATP-binding protein [EC: 3.6.3.27] (inferred from 86% identity to vei:Veis_1290)

MetaCyc: 74% identical to phosphate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.27

Use Curated BLAST to search for 3.6.3.27 or 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (266 amino acids)

>GFF4233 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MPNTATASAPAATAHAKNALELRNLNFFYGKFQGLKNVNMNIEERKVTAFIGPSGCGKST
LLRTLNRMYSLYPGQRAEGEINFYGQNILDSKQDINLLRARIGMVFQKPTPFPMSIYDNI
AFGVKLYEDLSRGEMDDRVEWALSKAALWNEVKDKLNQSGLSLSGGQQQRLCIARSVAVK
PSVLLLDEPTSALDPISTGKVEELVHELKQDYTIAIVTHNMQQAARCSDYTAYMYLGELI
EFGSTEQIFFKPNKTETEDYITGRFG