Protein Info for GFF4230 in Pseudomonas sp. DMC3

Annotation: Alpha-ketoglutarate-dependent sulfate ester dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 79% identical to ATSK_PSEPU: Alpha-ketoglutarate-dependent sulfate ester dioxygenase (atsK) from Pseudomonas putida

KEGG orthology group: K03119, taurine dioxygenase [EC: 1.14.11.17] (inferred from 94% identity to pfo:Pfl01_0213)

MetaCyc: 79% identical to type II alkyl sulfatase monomer (Pseudomonas putida)
RXN-17674 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]

Predicted SEED Role

"Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17)" in subsystem Alkanesulfonate assimilation or Taurine Utilization (EC 1.14.11.17)

MetaCyc Pathways

taurine degradation IV (1/1 steps found)
superpathway of taurine degradation (2/6 steps found)

KEGG Metabolic Maps

Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.14.11.17

Use Curated BLAST to search for 1.14.11.17 or 1.14.11.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (306 amino acids)

>GFF4230 Alpha-ketoglutarate-dependent sulfate ester dioxygenase (Pseudomonas sp. DMC3)
MSNAALAVKPVSHALEIHPVAGRIGAEIRGVHLSGELDAATVEAIQQALVEYKVLFFREQ
TQLDDQRQEAFAHLLGEPVAHPTVPSREGTRYLLELDGAEGQRANSWHTDVTFVDAYPKA
SILRSVVAPAFGGDTLWANTATAYSGLPSELRELADKLTAVHSNEYDYAGAKPDVSAEKL
ERYRKVFTSTVYETEHPVVRVHPISGEKSLLLGHFVKRIKGYSQADSAHLFGLLQSHVIR
QENTVRWRWQAGDVAIWDNRSTQHYAIDDYGTQDRVVRRVTLKGEVPVGVSGQRSQTIKG
PDIVGV